Fabrication of electrospun polycaprolactone coated withchitosan-silver nanoparticles membranes for wound dressing applications

  • Tra Thanh Nhi
  • Huynh Chan Khon
  • Nguyen Thi Thu Hoai
  • Bui Chi Bao
  • Tran Ngoc Quyen
  • Vo Van Toi
  • Nguyen Thi Hiep
Biomaterials Synthesis and Characterization Original Research
Part of the following topical collections:
  1. Biomaterials Synthesis and Characterization


In this study, electrospun polycaprolactone membrane coated with chitosan-silver nanoparticles (CsAg), electrospun polycaprolactone/chitosan/Ag nanoparticles, was fabricated by immersing the plasma-treated electrospun polycaprolactone membrane in the CsAg gel. The plasma modification of electrospun polycaprolactone membrane prior to CsAg coating was tested by methylene blue stain and scanning electron microscope. The presence of silver and chitosan on the plasma-treated electrospun polycaprolactone membrane was confirmed by energy-dispersive X-ray spectroscopy and FT-IR spectrum. Scanning electron microscope observation was employed to observe the morphology of the membranes. The release of Ag ions from electrospun polycaprolactone/chitosan/Ag nanoparticles membrane was tested using atomic absorption spectrometry. Electrospun polycaprolactone/chitosan/Ag nanoparticles membrane inherited advantages from both CsAg gel and electrospun polycaprolactone membrane such as: increasing biocompatibility, mechanical strength, and antibacterial activity against both Gram-negative and Gram-positive bacteria. Thus, this investigation introduces a highly potential membrane that can increase the efficacy of the wound dressing process.



The work relative with the PCL electrospun membranes is funded by Office of Navy Research (ONR) under grant number: N62909-14-1-N011-P00001, PR No: N6290914PR00015/N00014. The work relative with chitosan-AgNPs gel is funded by Vietnam National University-Ho Chi Minh City under grant number: B2013-76-03.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


  1. 1.
    Branco da Cunha C, et al. Influence of the stiffness of three-dimensional alginate/collagen-I interpenetrating networks on fibroblast biology. Biomaterials. 2014;35(32):8927–36.CrossRefGoogle Scholar
  2. 2.
    Boateng JS, et al. Wound healing dressings and drug delivery systems: a review. J Pharm Sci. 2008;97(8):2892–923.CrossRefGoogle Scholar
  3. 3.
    Liao N, et al. Electrospun bioactive poly (ɛ-caprolactone)–cellulose acetate–dextran antibacterial composite mats for wound dressing applications. Colloid Surface A. 2015;469:194–201.CrossRefGoogle Scholar
  4. 4.
    Chitrattha S, Phaechamud T. Porous poly(dl-lactic acid) matrix film with antimicrobial activities for wound dressing application. Mat Sci Eng C. 2016;58:1122–30.CrossRefGoogle Scholar
  5. 5.
    Liu S-J, et al. Electrospun PLGA/collagen nanofibrous membrane as early-stage wound dressing. J Memb Sci. 2010;355(1–2):53–9.CrossRefGoogle Scholar
  6. 6.
    Kevadiya BD, et al. Biodegradable gelatin–ciprofloxacin–montmorillonite composite hydrogels for controlled drug release and wound dressing application. Colloids Surf B. 2014;122:175–83.CrossRefGoogle Scholar
  7. 7.
    Duan K, et al. One-step synthesis of amino-reserved chitosan-graft-polycaprolactone as a promising substance of biomaterial. Carbohydr Polym. 2010;80(2):498–503.CrossRefGoogle Scholar
  8. 8.
    Nguyen T-H, Lee K-H, Lee B-T. Fabrication of Ag nanoparticles dispersed in PVA nanowire mats by microwave irradiation and electro-spinning. Mat Sci Eng C. 2010;30(7):944–50.CrossRefGoogle Scholar
  9. 9.
    Tran PA, Hocking DM, O’Connor AJ. In situ formation of antimicrobial silver nanoparticles and the impregnation of hydrophobic polycaprolactone matrix for antimicrobial medical device applications. Mat Sci Eng C. 2015;47:63–9.CrossRefGoogle Scholar
  10. 10.
    Nguyen TH, et al. Nano Ag loaded PVA nano-fibrous mats for skin applications. J Biomed Mater Res B Appl Biomater. 2011;96(2):225–33.CrossRefGoogle Scholar
  11. 11.
    Goy RC, Britto Dd, Assis OBG. A review of the antimicrobial activity of chitosan. Polímeros. 2009;19:241–47.CrossRefGoogle Scholar
  12. 12.
    Abdelgawad AM, Hudson SM, Rojas OJ. Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol) systems. Carbohydr Polym. 2014;100(0):166–78.CrossRefGoogle Scholar
  13. 13.
    Akmaz S, et al. The effect of Ag content of the chitosan-silver nanoparticle composite Material on the Structure and antibacterial activity. Adv Mater Sci Eng. 2013;2013:6.CrossRefGoogle Scholar
  14. 14.
    Sámano-Valencia, et al. Characterization and biocompatibility of chitosan gels with silver and gold nanoparticles. J Nanomater. 2014. 2014: 11.Google Scholar
  15. 15.
    Ojeda-Martínez ML, et al., Skin wound healing with chitosan thin films containing supported silver nanospheres. J Bioact Compat Polym, 2015;30:6617–632.Google Scholar
  16. 16.
    Nguyen TH, et al. A hybrid electrospun PU/PCL scaffold satisfied the requirements of blood vessel prosthesis in terms of mechanical properties, pore size, and biocompatibility. J Biomater Sci Polym Ed. 2013;24(14):1692–706. doi: 10.1080/09205063.2013.792642 Epub 2013 Apr 29CrossRefGoogle Scholar
  17. 17.
    Wu X, et al. The release properties of silver ions from Ag-nHA/TiO2/PA66 antimicrobial composite scaffolds. Biomed Mater. 2010;5(4):044105.CrossRefGoogle Scholar
  18. 18.
    Croisier F, et al. Polysaccharide-coated PCL nanofibers for wound dressing applications. Adv Healthc Mater. 2014;3(12):2032–9.CrossRefGoogle Scholar
  19. 19.
    Song W, et al. Coaxial PCL/PVA electrospun nanofibers: osseointegration enhancer and controlled drug release device. Biofabrication. 2013;5(3):035006.CrossRefGoogle Scholar
  20. 20.
    Gautam S, et al. Surface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering. Mater Sci Eng C. 2014;34:402–9.CrossRefGoogle Scholar
  21. 21.
    Martins A, et al. Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance. Small 2009;5(10):1195–206.Google Scholar
  22. 22.
    Mohiti-Asli M, Pourdeyhimi B, Loboa EG. Novel, silver-ion-releasing nanofibrous scaffolds exhibit excellent antibacterial efficacy without the use of silver nanoparticles. Acta Biomater. 2014;10(5):2096–104.CrossRefGoogle Scholar
  23. 23.
    Nguyen, T.T.C., et al. Highly lipophilic pluronics-conjugated polyamidoamine dendrimer nanocarriers as potential delivery system for hydrophobic drugs. Mater Sci Eng C.Google Scholar
  24. 24.
    Hoang Phuc D, et al. Fabrication of hyaluronan-poly(vinylphosphonic acid)-chitosan hydrogel for wound healing application. Int J Polym Sc. 2016;2016:9Google Scholar
  25. 25.
    Gautam S, et al. Fabrication and characterization of PCL/gelatin/chitosan ternary nanofibrous composite scaffold for tissue engineering applications. J Mater Sci. 2014;49(3):1076–89.CrossRefGoogle Scholar
  26. 26.
    Zhu Y, Gao C, Shen J. Surface modification of polycaprolactone with poly(methacrylic acid) and gelatin covalent immobilization for promoting its cytocompatibility. Biomaterials. 2002;23(24):4889–95.CrossRefGoogle Scholar
  27. 27.
    Lischer S, et al. Antibacterial burst-release from minimal Ag-containing plasma polymer coatings. J R Soc Interface. 2011;8(60):1019–30.CrossRefGoogle Scholar
  28. 28.
    Yohe ST, et al. 3D superhydrophobic electrospun meshes as reinforcement materials for sustained local drug delivery against colorectal cancer cells. J Control Release. 2012;162(1):92–101.CrossRefGoogle Scholar
  29. 29.
    Chu PK. Enhancement of surface properties of biomaterials using plasma-based technologies. SurfCoat Tech. 2007;201(19–20):8076–82.Google Scholar
  30. 30.
    Fayaz AM, et al. Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomedicine. 2010;6(1):103–9.CrossRefGoogle Scholar
  31. 31.
    Nguyen T-H, Lee B-T. In vitro and in vivo studies of rhBMP2-coated PS/PCL fibrous scaffolds for bone regeneration. J Biomed Mater Res A. 2013;101A(3):797–808.CrossRefGoogle Scholar
  32. 32.
    Zaman HU, et al. Physico-mechanical properties of wound dressing material and its biomedical application. J Mech Behav Biomed Mater. 2011;4(7):1369–75.CrossRefGoogle Scholar
  33. 33.
    Shevchenko RV, James SL, James SE. A review of tissue-engineered skin bioconstructs available for skin reconstruction. J R Soc Interface. 2010;7(43):229–58.CrossRefGoogle Scholar
  34. 34.
    Rigo C, et al. Active silver nanoparticles for wound healing. Int J Mol Sci. 2013;14(3):4817–40.CrossRefGoogle Scholar
  35. 35.
    Fish DN. Meropenem in the treatment of complicated skin and soft tissue infections. Ther Clin Risk Manag. 2006;2(4):401–15.CrossRefGoogle Scholar
  36. 36.
    Riss TL, Moravec RA, Niles AL, Duellman S, Benink H, Worzella TJ, Minor L. Cell Viability Assays. In: Sittampalam GS, Coussens NP, Nelson H, et al., editors. Assay Guidance Manual. Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004.Google Scholar
  37. 37.
    Darby IA, et al. Fibroblasts and myofibroblasts in wound healing. Clin, Cosmet Invest Dermatol. 2014;7:301–11.Google Scholar
  38. 38.
    Svendsen MN, et al. Bacterial antigen induced release of soluble vascular endothelial growth factor (VEGF) and VEGFR1 before and after surgery. Scand J Clin Lab Invest. 2005;65(3):237–47.CrossRefGoogle Scholar
  39. 39.
    Xue ML, Thakur A, Willcox M. Macrophage inflammatory protein-2 and vascular endothelial growth factor regulate corneal neovascularization induced by infection with Pseudomonas aeruginosa in mice. Immunol Cell Biol. 2002;80(4):323–7.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Tra Thanh Nhi
    • 1
  • Huynh Chan Khon
    • 1
  • Nguyen Thi Thu Hoai
    • 2
  • Bui Chi Bao
    • 3
  • Tran Ngoc Quyen
    • 4
  • Vo Van Toi
    • 1
  • Nguyen Thi Hiep
    • 1
  1. 1.Tissue Engineering and Regenerative Medicine Group, Department of Biomedical EngineeringInternational University, Vietnam National University-Ho Chi Minh City (VNU-HCM)HCMCVietnam
  2. 2.School of BiotechnologyInternational University, Vietnam National University-Ho Chi Minh City (VNU-HCM)HCMCVietnam
  3. 3.The Center for Molecular BiomedicineUniversity of Medicine and PharmacyHCMCVietnam
  4. 4.Department - Materiasl and Pharmaceutical chemistry, Institute of Applied Materials Science-Vietnam Academy of Science and TechnologyVASTHCMCVietnam

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