Three-dimensional polymer coated 45S5-type bioactive glass scaffolds seeded with human mesenchymal stem cells show bone formation in vivo

  • Fabian Westhauser
  • Christian Weis
  • Matthäus Prokscha
  • Leonie A. Bittrich
  • Wei Li
  • Kai Xiao
  • Ulrich Kneser
  • Hans-Ulrich Kauczor
  • Gerhard Schmidmaier
  • Aldo R. Boccaccini
  • Arash Moghaddam
Tissue Engineering Constructs and Cell Substrates Original Research
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates

Abstract

45S5-type bioactive glasses are a promising alternative to established substitutes for the treatment of bone defects. Because the three-dimensional (3D) structure of bone substitutes is crucial for bone ingrowth and formation, we evaluated the osteoinductive properties of different polymer coated 3D-45S5 bioactive glass (BG) scaffolds seeded with human mesenchymal stem cells (hMSC) in vivo. BG scaffolds coated with gelatin, cross-linked gelatin, and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) were seeded with hMSC prior to implantation into severe combined immunodeficiency mice. Newly formed bone was evaluated with histomorphometry and micro-computed tomography. Bone formation was detectable in all groups, whereas the gelatin-coated BG scaffolds showed the best results and should be considered in further studies.

Graphical Abstract

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Fabian Westhauser
    • 1
  • Christian Weis
    • 2
  • Matthäus Prokscha
    • 1
  • Leonie A. Bittrich
    • 3
  • Wei Li
    • 4
  • Kai Xiao
    • 1
  • Ulrich Kneser
    • 3
  • Hans-Ulrich Kauczor
    • 2
  • Gerhard Schmidmaier
    • 1
  • Aldo R. Boccaccini
    • 4
  • Arash Moghaddam
    • 1
  1. 1.HTRG - Heidelberg Trauma Research Group, Trauma and Reconstructive Surgery, Center of Orthopedics, Traumatology, and Spinal Cord InjuryHeidelberg University HospitalHeidelbergGermany
  2. 2.Clinic of Diagnostic and Interventional Radiology (DIR)Heidelberg University HospitalHeidelbergGermany
  3. 3.Clinic for Hand-, Plastic- and Reconstructive SurgeryBG-Unfallklinik LudwigshafenLudwigshafen am RheinGermany
  4. 4.Institute of BiomaterialsUniversity of Erlangen-NurembergErlangenGermany

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