Three-dimensional polymer coated 45S5-type bioactive glass scaffolds seeded with human mesenchymal stem cells show bone formation in vivo
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45S5-type bioactive glasses are a promising alternative to established substitutes for the treatment of bone defects. Because the three-dimensional (3D) structure of bone substitutes is crucial for bone ingrowth and formation, we evaluated the osteoinductive properties of different polymer coated 3D-45S5 bioactive glass (BG) scaffolds seeded with human mesenchymal stem cells (hMSC) in vivo. BG scaffolds coated with gelatin, cross-linked gelatin, and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) were seeded with hMSC prior to implantation into severe combined immunodeficiency mice. Newly formed bone was evaluated with histomorphometry and micro-computed tomography. Bone formation was detectable in all groups, whereas the gelatin-coated BG scaffolds showed the best results and should be considered in further studies.
KeywordsBone Formation Bioactive Glass PHBV Human Mesenchymal Stem Cell Severe Combine Immunodeficiency
The authors thank Tyler Swing for proofreading, Tom Bruckner for the support according the statistical analysis, and Birgit Frey for histomorphometric processing. This study was financed by the research grant of the Center of Orthopedics, Traumatology, and Spinal Cord Injury, Heidelberg University Hospital.
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