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Constructing bio-layer of heparin and type IV collagen on titanium surface for improving its endothelialization and blood compatibility

Journal of Materials Science: Materials in Medicine volume 27, Article number: 81 (2016) Cite this article

Abstract

The modification of cardiovascular stent surface for a better micro-environment has gradually changed to multi-molecule, multi-functional designation. In this study, heparin (Hep) and type IV collagen (IVCol) were used as the functional molecule to construct a bifunctional micro-environment of anticoagulation and promoting endothelialization on titanium (Ti). The surface characterization results (AFM, Alcian Blue 8GX Staining and fluorescence staining of IVCol) indicated that the bio-layer of Hep and IVCol were successfully fabricated on the Ti surface through electrostatic self-assembly. The APTT and platelet adhesion test demonstrated that the bionic layer possessed better blood compatibility compared with Ti surface. The adhesion, proliferation, migration and apoptosis tests of endothelial cells proved that the Hep/IVCol layer was able to enhance the endothelialization of the Ti surface. The in vivo animal implantation results manifested that the bionic surface could encourage new endothelialization. This work provides an important reference for the construction of multifunction micro-environment on the cardiovascular scaffold surface.

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Acknowledgments

This work was financially supported by the Funds of Key Basic Research Program (2011CB606204), the Joint Fund for Fostering Talents of National Natural Science Foundation of China and Henan province (U1504310), China Postdoctoral Science Foundation (2014M562333, 2015M582206), and Postdoctoral Scientific Research Fund of Henan Province (2014020).

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Authors and Affiliations

  1. School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People’s Republic of China

    Kun Zhang & Fang-xia Guan

  2. Center of Stem Cell and Regenerative Medicine, First Affiliated Hospital of Zhengzhou University, 40 University Road, Zhengzhou, 450052, People’s Republic of China

    Kun Zhang & Fang-xia Guan

  3. Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People’s Republic of China

    Kun Zhang, Jun-ying Chen, Jing-an Li & Nan Huang

  4. Jiangsu Heze Allian Cells Bioscience Co., Ltd, Changzhou, 213000, People’s Republic of China

    Wei Qin

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  1. Kun Zhang
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Correspondence to Kun Zhang.

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Zhang, K., Chen, Jy., Qin, W. et al. Constructing bio-layer of heparin and type IV collagen on titanium surface for improving its endothelialization and blood compatibility. J Mater Sci: Mater Med 27, 81 (2016). https://doi.org/10.1007/s10856-016-5693-6

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  • DOI: https://doi.org/10.1007/s10856-016-5693-6

Keywords

  • Activate Partial Thromboplastin Time
  • Collagen Coating
  • Blood Compatibility
  • Platelet Adhesion Test
  • Fluid Flow Shear Stress