Efficacy of silicate-substituted calcium phosphate with enhanced strut porosity as a standalone bone graft substitute and autograft extender in an ovine distal femoral critical defect model
- 536 Downloads
A synthetic bone graft substitute consisting of silicate-substituted calcium phosphate with increased strut porosity (SiCaP EP) was evaluated in an ovine distal femoral critical sized metaphyseal defect as a standalone bone graft, as an autologous iliac crest bone graft (ICBG) extender (SiCaP EP/ICBG), and when mixed with bone marrow aspirate (SiCaP EP/BMA). Defects were evaluated after 4, 8, and 12 weeks with radiography, decalcified paraffin-embedded histopathology, non-decalcified resin-embedded histomorphometry, and mechanical indentation testing. All test groups exhibited excellent biocompatibility and osseous healing as evidenced by an initial mild inflammatory response followed by neovascularization, bone growth, and marrow infiltration throughout all SiCaP EP-treated defects. SiCaP EP/ICBG produced more bone at early time points, while all groups produced similar amounts of bone at later time points. SiCaP EP/ICBG likewise showed more favorable mechanical properties at early time points, but was equivalent to SiCaP EP and SiCaP EP/BMA at later time points. This study demonstrates that SiCaP EP is efficacious as a standalone bone graft substitute, mixed with BMA, and as an autograft extender.
KeywordsBone Graft Bone Marrow Aspirate Bone Graft Substitute Iliac Crest Bone Graft Ultimate Compressive Strength
This research study was funded by Baxter Healthcare.
- 14.Fredericks DC, Petersen EB, Saihi N, Corley KGN, DeVries N, Grosland NM, Smucker JD. Evaluation of a novel silicate substituted hydroxyapatite bone graft substitute in a rabbit posterolateral fusion model. Iowa Orthop J. 2013;33:25–32.Google Scholar
- 15.Fredericks DC, Petersen EB, Al-Hilli A, Nepola JV, Gandhi AA, Kode S, Grosland NM, Smucker JD. In: Assessment of silicate-substituted calcium phosphate bone graft in a rabbit posterolateral fusion model with concurrent chemotherapy. Transactions of the 58th Annual Meeting of the Orthopaedic Research Society, 2012; p. 1112.Google Scholar
- 16.Coathup M, Samizadeh S, Amogbokpa J, Fang SY, Hing KA, Buckland T, Blunn GW. The osteoinductivity of silicon substituted hydroxyapatite. J Bone Joint Surg Am. 2011;93A(23):2219–26.Google Scholar
- 20.Chiang YM, Birnie DP III, Kingery WD. Physical ceramics: principles for ceramic science and engineering. New York: Wiley; 1997.Google Scholar
- 28.Pearce AI, Richards RG, Milz S, Schneider E, Pearce SG. Animal models for implant biomaterial research in bone: a review. Eur Cell Mater. 2007;13:1–10.Google Scholar
- 29.Martini L, Fini M, Giavaresi G, Giardino R. Sheep model in orthopedic research: a literature review. Comp Med. 2001;51(4):292–9.Google Scholar