Skip to main content

Inhibition of pathogenic bacterial growth on excision wound by green synthesized copper oxide nanoparticles leads to accelerated wound healing activity in Wistar Albino rats

Abstract

An impaired wound healing is one of the major health related problem in diabetic and non-diabetic patients around the globe. The pathogenic bacteria play a predominant role in delayed wound healing, owing to interaction in the wound area. In our previous work we developed green chemistry mediated copper oxide nanoparticles using Ficus religiosa leaf extract. In the present study we make an attempt to evaluate the anti-bacterial, and wound healing activity of green synthesized copper oxide nanoparticles in male Wistar Albino rats. The agar well diffusion assay revealed copper oxide nanoparticles have substantial inhibition activity against human pathogenic strains such as Klebsiella pneumoniae, Shigella dysenteriae, Staphylococcus aureus, Salmonella typhimurium and Escherichia coli, which were responsible for delayed wound healing process. Furthermore, the analyses results of wound closure, histopathology and protein profiling confirmed that the F. religiosa leaf extract tailored copper oxide nanoparticles have enhanced wound healing activity in Wistar Albino rats.

Graphical Abstract

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. 1.

    Sankar R, Dhivya R, Shivashangari KS, Ravikumar V. Wound healing activity of Origanum vulgare engineered titanium dioxide nanoparticles in Wistar Albino rats. J Mater Sci Mater Med. 2014;25:1701–8.

    Article  Google Scholar 

  2. 2.

    Sen CK, Gordillo GM, Roy S, Kirsner R, Lambert L, Hunt TK, Gottrup F, Gurtner GC, Longaker MT. Human skin wounds: a major and snowballing threat to public health and the economy. Wound Repair Regen. 2009;17:763–71.

    Article  Google Scholar 

  3. 3.

    Martin P. Wound healing-aiming for perfect skin regeneration. Science. 1997;276:75–81.

    Article  Google Scholar 

  4. 4.

    Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev. 2001;14:244–69.

    Article  Google Scholar 

  5. 5.

    Paphitou NI. Antimicrobial resistance: action to combat the rising microbial challenges. Int J Antimicrob Agents. 2013;42(Suppl):S25–8.

    Article  Google Scholar 

  6. 6.

    Sankar R, Karthik A, Prabu A, Karthik S, Shivashangari KS, Ravikumar V. Origanum vulgare mediated biosynthesis of silver nanoparticles for its antibacterial and anticancer activity. Colloids Surf B Biointerfaces. 2013;108:80–4.

    Article  Google Scholar 

  7. 7.

    Sankar R, Prasath BB, Nandakumar R, Santhanam P, Shivashangari KS, Ravikumar V. Growth inhibition of bloom forming cyanobacterium Microcystis aeruginosa by green route fabricated copper oxide nanoparticles. Environ Sci Pollut Res Int. 2014;21:14232–40.

    Article  Google Scholar 

  8. 8.

    Barathmanikanth S, Kalishwaralal K, Sriram M, Pandian SR, Youn HS, Eom S, Gurunathan S. Anti-oxidant effect of gold nanoparticles restrains hyperglycemic conditions in diabetic mice. J Nanobiotechnology. 2010;8:16.

    Article  Google Scholar 

  9. 9.

    Sankar R, Maheswari R, Karthik S, Shivashangari KS, Ravikumar V. Anticancer activity of Ficus religiosa engineered copper oxide nanoparticles. Mater Sci Eng C Mater Biol Appl. 2014;44:234–9.

    Article  Google Scholar 

  10. 10.

    Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic A. Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study. Int J Nanomedicine. 2012;7:6003–9.

    Article  Google Scholar 

  11. 11.

    Prabhu S, Poulose EK. Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int Nano Lett. 2012;2:32.

    Article  Google Scholar 

  12. 12.

    Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, Kim SH, Park YK, Park YH, Hwang CY, Kim YK, Lee YS, Jeong DH, Cho MH. Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007;3:95–101.

    Article  Google Scholar 

  13. 13.

    Chatterjee AK, Chakraborty R, Basu T. Mechanism of antibacterial activity of copper nanoparticles. Nanotechnology. 2014;25:135101.

    Article  Google Scholar 

  14. 14.

    Wilkinson LJ, White RJ, Chipman JK. Silver and nanoparticles of silver in wound dressings: a review of efficacy and safety. J Wound Care. 2011;20:543–9.

    Article  Google Scholar 

  15. 15.

    Cortivo R, Vindigni V, Iacobellis L, Abatangelo G, Pinton P, Zavan B. Nanoscale particle therapies for wounds and ulcers. Nanomedicine (Lond). 2010;5:641–56.

    Article  Google Scholar 

Download references

Acknowledgments

We are grateful to the Department of Science and Technology (DST) for providing the financial assistance to Mr. Renu Sankar through the INSPIRE Fellowship. We extend our acknowledgement to the University Grant Commission (UGC) and the Science & Engineering Research Board (SERB) for their financial support. We also thank the Department of Science and Technology-Fund for Improvement of S & T Infrastructure in Universities and Higher Educational Institutions (DST-FIST) for their infrastructure support to our department.

Author information

Affiliations

Authors

Corresponding authors

Correspondence to Kanchi Subramanian Shivashangari or Vilwanathan Ravikumar.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Sankar, R., Baskaran, A., Shivashangari, K.S. et al. Inhibition of pathogenic bacterial growth on excision wound by green synthesized copper oxide nanoparticles leads to accelerated wound healing activity in Wistar Albino rats. J Mater Sci: Mater Med 26, 214 (2015). https://doi.org/10.1007/s10856-015-5543-y

Download citation

Keywords

  • Metal Oxide Nanoparticles
  • Wound Contraction
  • Shigella Dysenteriae
  • Excision Wound
  • Copper Oxide Nanoparticles