The influence of SrO and CaO in silicate and phosphate bioactive glasses on human gingival fibroblasts
- 326 Downloads
In this paper, we investigate the effect of substituting SrO for CaO in silicate and phosphate bioactive glasses on the human gingival fibroblast activity. In both materials the presence of SrO led to the formation of a CaP layer with partial Sr substitution for Ca. The layer at the surface of the silicate glass consisted of HAP whereas at the phosphate glasses it was close to the DCPD composition. In silicate glasses, SrO gave a faster initial dissolution and a thinner reaction layer probably allowing for a continuous ion release into the solution. In phosphate glasses, SrO decreased the dissolution process and gave a more strongly bonded reaction layer. Overall, the SrO-containing silicate glass led to a slight enhancement in the activity of the gingival fibroblasts cells when compared to the SrO-free reference glass, S53P4. The cell activity decreased up to 3 days of culturing for all phosphate glasses containing SrO. Whereas culturing together with the SrO-free phosphate glass led to complete cell death at 7 days. The glasses containing SrO showed rapid cell proliferation and growth between 7 and 14 days, reaching similar activity than glass S53P4. The addition of SrO in both silicate and phosphate glasses was assumed beneficial for proliferation and growth of human gingival fibroblasts due to Sr incorporation in the reaction layer at the glass surface and released in the cell culture medium.
KeywordsSimulated Body Fluid Immersion Time Silicate Glass Bioactive Glass Phosphate Glass
The Academy of Finland is gratefully acknowledged for the financial support of Jonathan Massera.
- 2.Lindfors NC, Koski I, Heikkilä JT, Mattila K, Aho AJ. A prospective randomized 14-year follow-up study of bioactive glass and autogenous bone as bone graft substitutes in benign bone tumors. J Biomed Mater Res B Appl Biomater. 2010;94:157–64.Google Scholar
- 8.Reginster JY, Seeman E, DeVernejoul MC, Adami S, Compston J, Phenekos C, Devogelaer JP, Diaz Curiel M, Sawicki A, Goemaere S, Sorenson OH, Felsenberg D, Meunier PJ. Strontium ranelate reduces the risk of non-vertebral fractures in postmenopausal women with osteoporsis: Treatment of peripheral osteoporosis (TROPOS) study. J Clin Endocrinol Metab. 2005;90:2816–22.CrossRefGoogle Scholar
- 12.Isaac J, Nohra J, Lao J, Jallot E, Nedelec J-M, Berdal A, Sautier J-M. Effects of strontium-doped bioactive glass on the differentiation of cultured osteogenic cells. Eur Cells Mater. 2011;21:130–43.Google Scholar