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Assessment of human gingival fibroblast interaction with dental implant abutment materials

  • Vygandas RutkunasEmail author
  • Virginija Bukelskiene
  • Vaidotas Sabaliauskas
  • Evaldas Balciunas
  • Mangirdas Malinauskas
  • Daiva Baltriukiene
Tissue Engineering Constructs and Cell Substrates
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates

Abstract

The biocompatibility of dental implant abutment materials depends on numerous factors including the nature of the material, its chemical composition, roughness, texture, hydrophilicity and surface charge. The aim of the present study was to compare the viability and adhesion strength of human gingival fibroblasts (HGFs) grown on several dental materials used in implant prosthodontics. Surfaces of the tested materials were assessed using an optical imaging profiler. For material toxicity and cellular adhesion evaluation, primary human gingival fibroblast cells were used. To evaluate the strength of cellular adhesion, gingival fibroblasts were cultured on the tested materials and subjected to lateral shear forces by applying 300 and 500 rpm shaking intensities. Focal adhesion kinase (FAK) expression and phosphorylation in cells grown on the specimens were registered by cell-based ELISA. There was a tendency of fibroblast adhesion strength to decrease in the following order: sandblasted titanium, polished titanium, sandblasted zirconium oxide, polished zirconium oxide, gold–alloy, chrome–cobalt alloy. Higher levels of total as well as phospho-FAK protein were registered in HGFs grown on roughened titanium. Material type and surface processing technique have an impact on gingival fibroblast interaction with dental implant abutment materials.

Keywords

Focal Adhesion Kinase Adhesion Strength Gold Alloy Gingival Fibroblast Human Gingival Fibroblast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by Research Council of Lithuania, Grant No. MIP-11369.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Vygandas Rutkunas
    • 1
    Email author
  • Virginija Bukelskiene
    • 2
  • Vaidotas Sabaliauskas
    • 3
  • Evaldas Balciunas
    • 2
    • 4
  • Mangirdas Malinauskas
    • 4
  • Daiva Baltriukiene
    • 2
  1. 1.Department of Prosthodontics, Institute of Odontology, Faculty of MedicineVilnius UniversityVilniusLithuania
  2. 2.Department of Biological Models, Institute of BiochemistryVilnius UniversityVilniusLithuania
  3. 3.Vilnius University Hospital Zalgiris ClinicsVilniusLithuania
  4. 4.Laser Research Center, Department of Quantum Electronics, Faculty of PhysicsVilnius UniversityVilniusLithuania

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