Assessment of human gingival fibroblast interaction with dental implant abutment materials

  • Vygandas RutkunasEmail author
  • Virginija Bukelskiene
  • Vaidotas Sabaliauskas
  • Evaldas Balciunas
  • Mangirdas Malinauskas
  • Daiva Baltriukiene
Tissue Engineering Constructs and Cell Substrates
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates


The biocompatibility of dental implant abutment materials depends on numerous factors including the nature of the material, its chemical composition, roughness, texture, hydrophilicity and surface charge. The aim of the present study was to compare the viability and adhesion strength of human gingival fibroblasts (HGFs) grown on several dental materials used in implant prosthodontics. Surfaces of the tested materials were assessed using an optical imaging profiler. For material toxicity and cellular adhesion evaluation, primary human gingival fibroblast cells were used. To evaluate the strength of cellular adhesion, gingival fibroblasts were cultured on the tested materials and subjected to lateral shear forces by applying 300 and 500 rpm shaking intensities. Focal adhesion kinase (FAK) expression and phosphorylation in cells grown on the specimens were registered by cell-based ELISA. There was a tendency of fibroblast adhesion strength to decrease in the following order: sandblasted titanium, polished titanium, sandblasted zirconium oxide, polished zirconium oxide, gold–alloy, chrome–cobalt alloy. Higher levels of total as well as phospho-FAK protein were registered in HGFs grown on roughened titanium. Material type and surface processing technique have an impact on gingival fibroblast interaction with dental implant abutment materials.


Focal Adhesion Kinase Adhesion Strength Gold Alloy Gingival Fibroblast Human Gingival Fibroblast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported by Research Council of Lithuania, Grant No. MIP-11369.


  1. 1.
    Hallab NJ, Bundy KJ, O’Connor K, Moses RL, Jacobs JJ. Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion. Tissue Eng. 2001;7:55–71.CrossRefGoogle Scholar
  2. 2.
    Lange R, Luthen F, Beck U, Rychly J, Baumann A, Nebe B. Cell-extracellular matrix interaction and physico-chemical characteristics of titanium surfaces depend on the roughness of the material. Biomol Eng. 2002;19:255–61.CrossRefGoogle Scholar
  3. 3.
    Elias CN. Implant dentistry—a rapidly evolving practice. In: Turkyilmaz I. Factors affecting the success of dental implants. InTech; 2011. p 319–365.Google Scholar
  4. 4.
    Goreish HH, Lewis AL, Rose S, Lloyd AW. The effect of phosphorylcholine-coated materials on the inflammatory response and fibrous capsule formation: in vitro and in vivo observations. J Biomed Mater Res A. 2004;68:1–9.CrossRefGoogle Scholar
  5. 5.
    Wang YX, Robertson JL, Spillman WB, Claus RO. Effects of the chemical structure and the surface properties of polymeric biomaterials and their biocompatibility. Pharmaceut Res. 2004;21:1362–73.CrossRefGoogle Scholar
  6. 6.
    Thevenot P, Hu W, Tang L. Surface chemistry influences implant biocompatibility. Curr Top Med Chem. 2008;8:270–80.CrossRefGoogle Scholar
  7. 7.
    Wilson CJ, Clegg RE, Leavesley DI, Pearcy MJ. Mediation of biomaterial-cell interactions by adsorbed protein: a review. Tissue Eng. 2005;11:1–18.CrossRefGoogle Scholar
  8. 8.
    Akiyama SK. Integrins in cell adhesion and signaling. Hum Cell. 1996;9:181–6.Google Scholar
  9. 9.
    Gumbiner BM. Cell adhesion: the molecular basis of tissue architecture and morphogenesis. Cell. 1996;84:345–57.CrossRefGoogle Scholar
  10. 10.
    Burridge K, Chrzanowska-Wodnicka M. Focal adhesions, contractility, and signaling. Annu Rev Cell Dev Biol. 1996;12:463–519.CrossRefGoogle Scholar
  11. 11.
    Thamilselvan V, Craig DH, Basson MD. FAK association with multiple signal proteins mediates pressure-induced colon cancer cell adhesion via a Src-dependent PI3K/Akt pathway. FASEB J. 2007;21:1730–41.CrossRefGoogle Scholar
  12. 12.
    Schaller MD, Hildebrand JD, Parsons JT. Complex formation with focal adhesion kinase: a mechanism to regulate activity and subcellular localization of Src kinases. Mol Biol Cell. 1999;10:3489–505.CrossRefGoogle Scholar
  13. 13.
    Hanks SK, Ryzhova L, Shin NY, Brábek J. Focal adhesion kinase signaling activities and their implications in the control of cell survival and motility. Front Biosci. 2003;8:982–96.CrossRefGoogle Scholar
  14. 14.
    Schaller MD, Hildebrand JD, Shannon JD, Fox JW, Vines RR, Parsons JT. Autophosphorylation of the focal adhesion kinase pp125FAK, directs SH2-depentent binding of pp60src. Mol Biol Cell. 1994;14:1680–8.Google Scholar
  15. 15.
    Michael KE, Dumbauld DW, Burns KL, Hanks SK, García AJ. Focal adhesion kinase modulates cell adhesion strengthening via integrin activation. Mol Biol Cell. 2009;20:2508–19.CrossRefGoogle Scholar
  16. 16.
    Ilic D, Damsky CH, Yamamoto T. Focal adhesion kinase: at the crossroads of signal transduction. J Cell Sci. 1997;110:401–7.Google Scholar
  17. 17.
    Maxson BB, Syed SA, Dominguez BL. Clinical and microbiologic comparisons of two dental implant systems. Oral Surg Oral Diagn. 1992;3:31–5.Google Scholar
  18. 18.
    Buser D, Mericske-Stern R, Bernard JP, Behneke A, Behneke N, Hirt HP, Belser UC, Lang NP. Long-term evaluation of non-submerged ITI implants. Part 1: 8-year life table analysis of a prospective multi-center study with 2359 implants. Clin Oral Implants Res. 1997;8:161–72.CrossRefGoogle Scholar
  19. 19.
    Fartash B, Arvidson K. Long-term evaluation of single crystal sapphire implants as abutments in fixed prosthodontics. Clin Oral Implants Res. 1997;8:58–67.CrossRefGoogle Scholar
  20. 20.
    Cabral MCT, Costa MA, Fernandes MH. In vitro models of periodontal cells: a comparative study of long-term gingival, periodontal ligament and alveolar bone cell cultures in the presence of β-glycerophosphate and dexamethasone. J Mater Sci Mater Med. 2007;18:1079–88.CrossRefGoogle Scholar
  21. 21.
    Keller G, Sebastian J, Lacombe U, Toft K, Lask G, Revazova E. Safety of injectable autologous human fibroblasts. Bull Exp Biol Med. 2000;130:786–9.CrossRefGoogle Scholar
  22. 22.
    Rompen E, Domken O, Degidi M, Pontes AE, Piattelli A. The effect of material characteristics, of surface topography and of implant components and connections on soft tissue integration: a literature review. Clin Oral Implants Res. 2006;17:55–67.CrossRefGoogle Scholar
  23. 23.
    Welander M, Abrahamsson I, Berglundh T. The mucosal barrier at implant abutments of different materials. Clin Oral Implants Res. 2008;19:635–41.Google Scholar
  24. 24.
    Nevins M, Camelo M, Nevins ML, Schupbach P, Kim DM. Connective tissue attachment to laser-microgrooved abutments: a human histologic case report. Int J Prosthodont Restor Dent. 2012;32:385–92.Google Scholar
  25. 25.
    Jin C, Ren LF, Ding HZ, Shi GS, Lin HS, Zhang F. Enhanced attachment, proliferation, and differentiation of human gingival fibroblasts on titanium surface modified with biomolecules. J Biomed Mater Res B Appl Biomater. 2012;100:2167–77.CrossRefGoogle Scholar
  26. 26.
    Watanabe H, Saito K, Kokubun K, Sasaki H, Yoshinari M. Change in surface properties of zirconia and initial attachment of osteoblast like cells with hydrophilic treatment. Dent Mater J. 2012;31:806–14.CrossRefGoogle Scholar
  27. 27.
    Yazici H, Fong H, Wilson B, Oren EE, Amos FA, Zhang H, Evans JS, Snead ML, Sarikaya M, Tamerler C. Biological response on a titanium implant-grade surface functionalized with modular peptides. Acta Biomater. 2013;9:5341–52.CrossRefGoogle Scholar
  28. 28.
    Baltriukiene D, Sabaliauskas V, Balciunas E, Melninkaitis A, Liutkevicius E, Bukelskiene V, Rutkunas V. The effect of laser-treated titanium surface on human gingival fibroblast behavior. J Biomed Mater Res A. 2014;102:713–20.CrossRefGoogle Scholar
  29. 29.
    Moller B, Terheyden H, Acil Y, Purcz NM, Hertrampf K, Tabakov A, Behrens E, Wiltfang J. A comparison of biocompatibility and osseointegration of ceramic and titanium implants: an in vivo and in vitro study. Int J Oral Maxillofac Surg. 2012;41:638–45.CrossRefGoogle Scholar
  30. 30.
    Meretoja VV, Rossi S, Peltola T, Pelliniemi LJ, Närhi TO. Adhesion and proliferation of human fibroblasts on sol–gel coated titania. J Biomater Res A. 2010;95:269–75.Google Scholar
  31. 31.
    Ungersbock A, Pohler O, Perren SM. Evaluation of the soft tissue interface at titanium implants with different surface treatments: experimental study on rabbits. Bio-Med Mater Eng. 1994;4:317–25.Google Scholar
  32. 32.
    Kim H, Murakami H, Chehroudi B, Textor M, Brunette DM. Effects of surface topography on the connective tissue attachment to subcutaneous implants. Int J Oral Maxillofac Surg. 2006;21:354–65.Google Scholar
  33. 33.
    Eisenbarth E, Linez P, Biehl V, Velten D, Breme J, Hildebrand HF. Cell orientation and cytoskeleton organization on ground titanium surfaces. Biomol Eng. 2002;19:233–7.CrossRefGoogle Scholar
  34. 34.
    Miura S, Takebe J. Biological behavior of fibroblast-like cells cultured on anodized-hydrothermally treated titanium with a nanotopographic surface structure. J Prosthodont Res. 2012;56:178–86.CrossRefGoogle Scholar
  35. 35.
    Chehroudi B, Gould TR, Brunette DM. A light and electron microscopic study of the effects of surface topography on the behavior of cells attached to titanium-coated percutaneous implants. J Biomed Mater Res A. 1991;25:387–405.CrossRefGoogle Scholar
  36. 36.
    Chehroudi B, Gould TR, Brunette DM. The role of connective tissue in inhibiting epithelial downgrowth on titanium-coated percutaneous implants. J Biomed Mater Res. 1992;26:493–515.CrossRefGoogle Scholar
  37. 37.
    Kawahara H, Kawahara D, Hashimoto K, Takashima Y, Ong JL. Morphologic studies on the biologic seal of titanium dental implants. Report I. In vitro study on the epithelialization mechanism around the dental implant. Int J Oral Maxillofac Surg. 1998;13:457–64.Google Scholar
  38. 38.
    Kawahara H, Kawahara D, Mimura Y, Takashima Y, Ong JL. Morphologic studies on the biologic seal of titanium dental implants. Report II. In vivo study on the defending mechanism of epithelial adhesions/attachment against invasive factors. Int J Oral Maxillofac Surg. 1998;13:465–73.Google Scholar
  39. 39.
    Ponsonnet L, Comte V, Othmane A, Lagneau C, Charbonnie M, Lissac M, Jaffrezic N. Effect of surface topography and chemistry on adhesion, orientation and growth of fibroblasts on nickel–titanium substrates. Mater Sci Eng Part C. 2002;21:157–65.CrossRefGoogle Scholar
  40. 40.
    Cochran D, Simpson J, Weber HP, Buser D. Attachment and growth of periodontal cells on smooth and rough titanium. Int J Oral Maxillofac Surg. 1994;9:289–97.Google Scholar
  41. 41.
    Takamori ER, Cruz R, Goncalvez F, Zanetti RV, Zanetti A, Granjeiro JM. Effect of roughness of zirconia and titanium on fibroblast adhesion. Artif Organs. 2008;32:305–9.CrossRefGoogle Scholar
  42. 42.
    Huang HH, Ho CT, Lee TH, Lee TL, Liao KK, Chen FL. Effect of surface roughness of ground titanium on initial cell adhesion. Biomol Eng. 2004;21:93–7.CrossRefGoogle Scholar
  43. 43.
    Dunn GA, Brown AF. Alignment of fibroblasts on grooved surfaces described by a simple geometric transformation. J Cell Sci. 1986;83:313–40.Google Scholar
  44. 44.
    Kokoti M, Sivropoulou A, Koidis P, Garefis P. Comparison of cell proliferation on modified dental ceramics. J Oral Rehabil. 2001;28:880–7.CrossRefGoogle Scholar
  45. 45.
    Scotchford CA, Ball M, Winkelmann M, Voros J, Csucs C, Brunette DM, Danuser G, Textor M. Chemically patterned, metal-oxide-based surfaces produced by photolithographic techniques for studying protein- and cell-interactions. II: protein adsorption and early cell interactions. Biomaterials. 2003;24:1147–58.CrossRefGoogle Scholar
  46. 46.
    Abrahamsson I, Berglundh T, Glantz PO, Lindhe J. The mucosal attachment at different abutments. An experimental study in dogs. J Clin Periodontol. 1998;25:721–7.CrossRefGoogle Scholar
  47. 47.
    Andersson B, Taylor A, Lang BR, Scheller H, Scharer P, Sorensen JA, Tarnow D. Alumina ceramic implant abutments used for single-tooth replacement: a prospective 1- to 3-year multicenter study. Int J Prosthodont. 2001;14:432–8.Google Scholar
  48. 48.
    Anselme K, Linez P, Bigerelle M, Le Maguer D, Le Mague A, Hardouin P, Hildebrand HF, Iost A, Leroy JM. The relative influence of the topography and chemistry of TiAl6V4 surfaces on osteoblastic cell behaviour. Biomaterials. 2000;21:1567–77.CrossRefGoogle Scholar
  49. 49.
    Bachle M, Kohal RJ. A systematic review of the influence of different titanium surfaces on proliferation, differentiation and protein synthesis of osteoblast-like Mg63 cells. Clin Oral Implants Res. 2004;15:683–92.CrossRefGoogle Scholar
  50. 50.
    Meyle J. Cell adhesion and spreading on different implant surfaces. Proceedings of the 3rd European Workshop on Periodontology, Berlin 1999;55–72.Google Scholar
  51. 51.
    Grossner-Schreiber B, Herzog M, Hedderich J, Duck A, Hannig M, Griepentrog M. Focal adhesion contact formation by fibroblasts cultured on surface-modified dental implants: an in vitro study. Clin Oral Implants Res. 2006;17:736–45.CrossRefGoogle Scholar
  52. 52.
    Subramani K, Jung RE, Molenberg A, Hammerle CH. Biofilm on dental implants: a review of the literature. Int J Oral Maxillofac Surg. 2009;24:616–26.Google Scholar
  53. 53.
    Wennerberg A, Sennerby L, Kultje C, Lekholm U. Some soft tissue characteristics at implant abutments with different surface topography. A study in humans. J Clin Periodontol. 2003;30:88–94.CrossRefGoogle Scholar
  54. 54.
    Elter C, Heuer W, Demling A, Hannig M, Heidenblut T, Bach FW, Stiesch-Scholz M. Supra- and subgingival biofilm formation on implant abutments with different surface characteristics. Int J Oral Maxillofac Implants. 2008;23:327–34.Google Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Vygandas Rutkunas
    • 1
    Email author
  • Virginija Bukelskiene
    • 2
  • Vaidotas Sabaliauskas
    • 3
  • Evaldas Balciunas
    • 2
    • 4
  • Mangirdas Malinauskas
    • 4
  • Daiva Baltriukiene
    • 2
  1. 1.Department of Prosthodontics, Institute of Odontology, Faculty of MedicineVilnius UniversityVilniusLithuania
  2. 2.Department of Biological Models, Institute of BiochemistryVilnius UniversityVilniusLithuania
  3. 3.Vilnius University Hospital Zalgiris ClinicsVilniusLithuania
  4. 4.Laser Research Center, Department of Quantum Electronics, Faculty of PhysicsVilnius UniversityVilniusLithuania

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