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Osseointegration of titanium implants functionalised with phosphoserine-tethered poly(epsilon-lysine) dendrons: a comparative study with traditional surface treatments in sheep

  • Biomaterials Synthesis and Characterization
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Abstract

The aim of this study was to analyse the osseointegrative potential of phosphoserine-tethered dendrons when applied as surface functionalisation molecules on titanium implants in a sheep model after 2 and 8 weeks of implantation. Uncoated and dendron-coated implants were implanted in six sheep. Sandblasted and etched (SE) or porous additive manufactured (AM) implants with and without additional dendron functionalisation (SE-PSD; AM-PSD) were placed in the pelvic bone. Three implants per group were examined histologically and six implants were tested biomechanically. After 2 and 8 weeks the bone-to-implant contact (BIC) total values of SE implants (43.7 ± 12.2; 53.3 ± 9.0 %) and SE-PSD (46.7 ± 4.5; 61.7 ± 4.9 %) as well as AM implants (20.49 ± 5.1; 43.9 ± 9.7 %) and AM-PSD implants (19.7 ± 3.5; 48.3 ± 15.6 %) showed no statistically significant differences. For SE-PSD and AM-PSD a separate analysis of only the cancellous BIC demonstrated a statistically significant difference after 2 and 8 weeks. Biomechanical findings proved the overall increased stability of the porous implants after 8 weeks. Overall, the great effect of implant macro design on osseointegration was further supported by additional phosphoserine-tethered dendrons for SE and AM implants.

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Acknowledgments

This work has been supported by Eurocoating SpA through the Provincia Autonoma of Trento grant, Biosintering project.

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Authors and Affiliations

  1. Musculoskeletal Research Unit, Equine Hospital, Vetsuisse Faculty ZH, University of Zurich, Winterthurerstrasse 260, 8057, Zurich, Switzerland

    Stefan Stübinger, Katja Nuss, Isabel Mosch, Miché le Sidler & Brigitte von Rechenberg

  2. Center for Applied Biotechnology and Molecular Medicine (CABMM), Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland

    Stefan Stübinger & Brigitte von Rechenberg

  3. Institute for Surgical Biotechnology and Biomechanics, University of Bern, Stauffacherstrasse 78, 3014, Bern, Switzerland

    Alexander Bürki

  4. Brighton Studies in Tissue-mimicry and Aided Regeneration, Brighton Centre for Regenerative Medicine, University of Brighton, Huxley Building Lewes Road, Brighton, BN2 4GJ, UK

    Steve T. Meikle & Matteo Santin

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  1. Stefan Stübinger
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  2. Katja Nuss
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Correspondence to Stefan Stübinger.

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Stübinger, S., Nuss, K., Bürki, A. et al. Osseointegration of titanium implants functionalised with phosphoserine-tethered poly(epsilon-lysine) dendrons: a comparative study with traditional surface treatments in sheep. J Mater Sci: Mater Med 26, 87 (2015). https://doi.org/10.1007/s10856-015-5433-3

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