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Influence of matrix and filler fraction on biofilm formation on the surface of experimental resin-based composites

  • Biomaterials Synthesis and Characterization
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Abstract

The aim of this study was to investigate the impact of resin matrix chemistry and filler fraction on biofilm formation on the surface of experimental resin-based composites (RBCs). Specimens were prepared from eight experimental RBC formulations differing in resin matrix blend (BisGMA/TEGDMA in a 7:3 wt% ratio or UDMA/aliphatic dimethacrylate in a 1:1 wt% ratio) and filler fraction (no fillers; 65 wt% dental glass with an average diameter of 7 or 0.7 µm or 65 wt% SiO2 with an average diameter of 20 nm). Surface roughness, surface free energy, and chemical surface composition were determined; surface topography was visualized using atomic force microscopy. Biofilm formation was simulated under continuous flow conditions for a 48 h period using a monospecies Streptococcus mutans and a multispecies biofilm model. In the monospecies biofilm model, the impact of the filler fraction overruled the influence of the resin matrix, indicating lowest biofilm formation on RBCs with nano-scaled filler particles and those manufactured from the neat resin blends. The multispecies model suggested a more pronounced effect of the resin matrix blend, as significantly higher biofilm formation was identified on RBCs with a UDMA/dimethacrylate matrix blend than on those including a BisGMA/TEGDMA matrix blend but analogous filler fractions. Although significant differences in surface properties between the various materials were identified, correlations between the surface properties and biofilm formation were poor, which highlights the relevance of surface topography and chemistry. These results may help to tailor novel RBC formulations which feature reduced biofilm formation on their surface.

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Acknowledgments

The authors would like to thank VOCO (Cuxhaven, Germany) and in particular Dr. Reinhard Maletz for financially supporting the study and for providing the different experimental RBCs. The entire work was performed without any intervention from VOCO.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy

    Andrei Ionescu, Eugenio Brambilla & Gloria Cazzaniga

  2. Institute of Experimental and Applied Physics, University of Regensburg, 93040, Regensburg, Germany

    Daniel S. Wastl & Franz J. Giessibl

  3. Department of Prosthetic Dentistry, Regensburg University Medical Center, 93042, Regensburg, Germany

    Sibylle Schneider-Feyrer & Sebastian Hahnel

Authors
  1. Andrei Ionescu
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  2. Eugenio Brambilla
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  3. Daniel S. Wastl
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  4. Franz J. Giessibl
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  5. Gloria Cazzaniga
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  6. Sibylle Schneider-Feyrer
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  7. Sebastian Hahnel
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Correspondence to Sebastian Hahnel.

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Ionescu, A., Brambilla, E., Wastl, D.S. et al. Influence of matrix and filler fraction on biofilm formation on the surface of experimental resin-based composites. J Mater Sci: Mater Med 26, 58 (2015). https://doi.org/10.1007/s10856-014-5372-4

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  • DOI: https://doi.org/10.1007/s10856-014-5372-4

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