Fibrin network architectures in pure platelet-rich plasma as characterized by fiber radius and correlated with clotting time


Fibrin networks are obtained through activation of platelet-rich plasma (PRP) for use in tissue regeneration. The importance of fibrin networks relies on mediation of release of growth factors, proliferation of tissue cells and rheological properties of the fibrin gels. Activation of PRP usually involves the decomposition of fibrinogen by agonists, in a wide range of concentrations. Therefore fibrin networks with a large structural diversity are formed, making comparative evaluations difficult. In order to standardize the fibrin networks, we used the statistical techniques central composite rotatable design and response-surface analysis, to correlate the radius of the fibers with the ratios between the agonists (autologous serum/calcium chloride) and agonist/PRP. From an individual and interactive analysis of the variables, architectures characterized by thick, medium and thin fibers were delineated on the response-surface. Furthermore, the architectures were correlated with coagulation time. This approach is valuable for standardizing the PRP preparation for clinical applications.

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The authors acknowledge the blood donors, and the Fundação de Amparo à Pesquisa do Estado de São Paulo for the financial support.

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Correspondence to Maria H. A. Santana.

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Perez, A.G.M., Rodrigues, A.A., Luzo, A.C.M. et al. Fibrin network architectures in pure platelet-rich plasma as characterized by fiber radius and correlated with clotting time. J Mater Sci: Mater Med 25, 1967–1977 (2014).

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  • Fibrinogen
  • Clotting Time
  • Fibrinogen Concentration
  • Autologous Serum
  • Central Composite Rotatable Design