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Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications

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Abstract

In this paper we describe the one-pot fabrication of hydroxyapatite (HA)-heparin composites by electrodeposition onto Ti substrates and their characterisation in terms of structure, morphology, heparin content and bioactivity. HA coatings are well known and widely applied osteointegration enhancers, but post-implant healing rate in dental applications is still suboptimal: e.g. coagulation control plays a key role and the incorporation of an anticoagulant is considered a highly desirable option. In this study, we have developed an improved, simple and robust growth procedure for single-phase, pure HA-heparin films of thickness 1/3 μm. HA-heparin, forming nanowires, has the ideal morphology for bone mineralisation. Staining assays revealed homogeneous incorporation of sizable amounts of heparin in the composite films. The bioactivities of the HA and HA-heparin coatings on Ti were compared by HeLa cell proliferation/viability tests and found to be enhanced by the presence of the anticoagulant.

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Authors and Affiliations

  1. Dipartimento di Ingegneria dell’Innovazione, Università del Salento, via Monteroni, 73100, Lecce, Italy

    Benedetto Bozzini, Francesco Bogani, Paolo Carlino & Claudio Mele

  2. Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Università del Salento, via Monteroni, 73100, Lecce, Italy

    Amilcare Barca, Tiziano Verri & Alessandro Romano

  3. Dipartimento di Meccanica, Politecnico di Milano, via La Masa 1, 20156, Milan, Italy

    Marco Boniardi

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  1. Benedetto Bozzini
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Correspondence to Benedetto Bozzini.

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Bozzini, B., Barca, A., Bogani, F. et al. Electrodeposition of nanostructured bioactive hydroxyapatite-heparin composite coatings on titanium for dental implant applications. J Mater Sci: Mater Med 25, 1425–1434 (2014). https://doi.org/10.1007/s10856-014-5186-4

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