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The effects of micro arc oxidation of gamma titanium aluminide surfaces on osteoblast adhesion and differentiation

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Abstract

The adhesion and proliferation of human fetal osteoblasts, hFOB 1.19, on micro arc oxidized (MAO) gamma titanium aluminide (γTiAl) surfaces were examined in vitro. Cells were seeded on MAO treated γTiAl disks and incubated for 3 days at 33.5 °C and subsequently for 7 days at 39.5 °C. Samples were then analyzed by scanning electron microscopy (SEM) and alkaline phosphatase assay (ALP) to evaluate cell adhesion and differentiation, respectively. Similar Ti-6Al-4V alloy samples were used for comparison. Untreated γTiAl and Ti-6Al-4V disks to study the effect of micro arc oxidation and glass coverslips as cell growth controls were also incubated concurrently. The ALP Assay results, at 10 days post seeding, showed significant differences in cell differentiation, with P values <0.05 between MAO γTiAl and MAO Ti-6Al-4V with respect to the corresponding untreated alloys. While SEM images showed that hFOB 1.19 cells adhered and proliferated on all MAO and untreated surfaces, as well as on glass coverslips at 10 days post seeding, cell differentiation, determined by the ALP assay, was significantly higher for the MAO alloys.

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Acknowledgments

This project was supported by the Grant SO6GM-08103 from the National Institutes of Health (NIH), National Institute of General Medical Sciences (NIGMS)/MBRS-SCORE, University of Puerto Rico, Mayagüez Campus. The authors would also like to acknowledge Boris Renteria from the General Engineering Department at UPRM for help with the AFM and Jose Almodóvar from the Biology Department for help with the SEM imaging.

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Authors and Affiliations

  1. Department of Biology, University of Puerto Rico, Mayaguez, PR, 00680, USA

    Pricilla Santiago-Medina & Nanette Diffoot-Carlo

  2. Department of Mechanical Engineering, University of Puerto Rico, Mayaguez, PR, 00680, USA

    Paul A. Sundaram

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  1. Pricilla Santiago-Medina
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  2. Paul A. Sundaram
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  3. Nanette Diffoot-Carlo
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Correspondence to Nanette Diffoot-Carlo.

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Santiago-Medina, P., Sundaram, P.A. & Diffoot-Carlo, N. The effects of micro arc oxidation of gamma titanium aluminide surfaces on osteoblast adhesion and differentiation. J Mater Sci: Mater Med 25, 1577–1587 (2014). https://doi.org/10.1007/s10856-014-5179-3

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  • DOI: https://doi.org/10.1007/s10856-014-5179-3

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