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In vitro and transdermal penetration of PHBV micro/nanoparticles

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Abstract

The purpose of this study was to develop micro and nano sized drug carriers from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and study the cell and skin penetration of these particles. PHBV micro/nanospheres were prepared by o/w emulsion method and were stained with a fluorescent dye, Nile Red. The particles were fractionated by centrifugation to produce different sized populations. Topography was studied by SEM and average particle size and its distribution were determined with particle sizer. Cell viability assay (MTT) was carried out using L929 fibroblastic cell line, and particle penetration into the cells were studied. Transdermal permeation of PHBV micro/nanospheres and tissue reaction were studied using a BALB/c mouse model. Skin response was evaluated histologically and amount of PHBV in skin was determined by gas chromatography-mass spectrometry. The average diameters of the PHBV micro/nanosphere batches were found to be 1.9 μm, 426 and 166 nm. Polydispersity indices showed that the size distribution of micro sized particles was broader than the smaller ones. In vitro studies showed that the cells had a normal growth trend. MTT showed no signs of particle toxicity. The 426 and 166 nm sized PHBV spheres were seen to penetrate the cell membrane. The histological sections revealed no adverse effects. In view of this data nano and micro sized PHBV particles appeared to have potential to serve as topical and transdermal drug delivery carriers for use on aged or damaged skin or in cases of skin diseases such as psoriasis, and may even be used in gene transfer to cells.

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Acknowledgments

The study was supported by the Government of the Russian Federation (Decree No. 220 of 09.04.2010) (Agreement No. 11.G34.31.0013) and (Grant № MD-3112.2012.4). We gratefully acknowledge the EC FP7 SKINTREAT project and the State Planning Organization (Turkey) for the grant to establish BIOMATEN. Mr. A. Büyüksungur is acknowledged for his contributions with CLSM.

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Authors and Affiliations

  1. Department of Micro and Nanotechnology, Middle East Technical University, 06800, Ankara, Turkey

    G. Eke, N. Hasirci & V. Hasirci

  2. Department of Biotechnology, Middle East Technical University, 06800, Ankara, Turkey

    N. Hasirci & V. Hasirci

  3. Department of Biomedical Engineering, Middle East Technical University, 06800, Ankara, Turkey

    N. Hasirci & V. Hasirci

  4. Department of Chemistry, Middle East Technical University, 06800, Ankara, Turkey

    N. Hasirci

  5. Department of Biological Sciences, Middle East Technical University, 06800, Ankara, Turkey

    V. Hasirci

  6. BIOMATEN, METU Center of Excellence in Biomaterials and Tissue Engineering, Biotechnology Research Unit Bldg, 06800, Ankara, Turkey

    G. Eke, N. Hasirci & V. Hasirci

  7. Department of Chemistry, Faculty of Arts and Sciences, Ahi Evran University, 40100, Kirsehir, Turkey

    G. Eke

  8. Siberian Federal University, Svobodnyi Avenue, Krasnoyarsk, 660041, Russia

    A. M. Kuzmina & E. I. Shishatskaya

  9. Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk, 66003, Russia

    A. V. Goreva & E. I. Shishatskaya

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  1. G. Eke
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  2. A. M. Kuzmina
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  3. A. V. Goreva
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  4. E. I. Shishatskaya
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  5. N. Hasirci
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  6. V. Hasirci
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Correspondence to V. Hasirci.

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Eke, G., Kuzmina, A.M., Goreva, A.V. et al. In vitro and transdermal penetration of PHBV micro/nanoparticles. J Mater Sci: Mater Med 25, 1471–1481 (2014). https://doi.org/10.1007/s10856-014-5169-5

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  • DOI: https://doi.org/10.1007/s10856-014-5169-5

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