Differentiation of human adipose-derived stem cells seeded on mineralized electrospun co-axial poly(ε-caprolactone) (PCL)/gelatin nanofibers
Mineralized poly(ε-caprolactone)/gelatin core–shell nanofibers were prepared via co-axial electrospinning and subsequent incubation in biomimetic simulated body fluid containing ten times the calcium and phosphate ion concentrations found in human blood plasma. The deposition of calcium phosphate on the nanofiber surfaces was investigated through scanning electronic microscopy and X-ray diffraction. Energy dispersive spectroscopy results indicated that calcium-deficient hydroxyapatite had grown on the fibers. Fourier transform infrared spectroscopy analysis suggested the presence of hydroxyl-carbonate-apatite. The results of a viability assay (MTT) and alkaline phosphatase activity analysis suggested that these mineralized matrices promote osteogenic differentiation of human adipose-derived stem cells (hASCs) when cultured in an osteogenic medium and have the potential to be used as a scaffold in bone tissue engineering. hASCs cultured in the presence of nanofibers in endothelial differentiation medium showed lower rates of proliferation than cells cultured without the nanofibers. However, endothelial cell markers were detected in cells cultured in the presence of nanofibers in endothelial differentiation medium.
KeywordsEnergy Dispersive Spectroscopy Simulated Body Fluid Bone Tissue Engineering DCPD Amorphous Calcium Phosphate
The authors acknowledge the financial support from National Council for Scientific and Technological Development (CNPq), a foundation linked to the Ministry of Science and Technology (MCT) of the Brazilian Government and Coordination of Improvement of Senior Staff (CAPES).
- 6.Paula ACC, Zonari AAC, Martins TMM, Novikoff S, Silva ARP, Correlo VM, Reis RL, Gomes DA, Goes AM. Human serum is a suitable supplement for the osteogenic differentiation of human adipose-derived stem cells seeded on poly-3-hydroxibutyrate-co-3-hydroxyvalerate scaffolds. Tissue Eng Part A. 2012;19(1–2):277–89.Google Scholar
- 30.Pereira IHL. Structural characterization of apatite like materials. Thesis submitted to the School of Metallurgy and Materials of Federal University of Minas Gerais (2013); pp 78. http://www.ppgem.eng.ufmg.br/diss.php Accessed 06 Dec 2013.
- 31.Ito A, Onuma K. Growth of Hydroxyapatite Crystals. Chaper 16 pp. 525-559. The on line version of: Crystal Growth Tecnology. Edited by: Kullaiah Byrappa, Tadashi Ohachi, Walter Michaeli, Hans Warlimont and Eicke Weber. ISBN: 978-0-8155-1453-4. 2003 William Andrew Inc. Accessed 12 Jan 2013.Google Scholar
- 35.Ribeiro Neto WA, Pereira IHL, Ayres E, Paula ACC, Averous L, Góes AM, Oréfice RL, Bretas RES. Influence of the microstructure and mechanical strength of nanofibers of biodegradable polymers with hydroxyapatite in stem cells growth. Electrospinning, characterization and cell viability. Polym Degrad Stab. 2012;97:2037–51.CrossRefGoogle Scholar
- 37.Correa FG, Martínez JB, Gómez JS. Synthesis and characterization of calcium phosphate and its relation to Cr(VI) adsorption properties. Rev Int Contam Ambient. 2010;26:129–34.Google Scholar
- 39.Li J. Structural characterization of apatite like materials. Thesis submitted to the School of Metallurgy and Materials of University of Birmingham (2009); pp 56-60. http://etheses.bham.ac.uk/618/1/Li10MRes.pdf Accessed 31 Jan 2013.
- 40.Franco PQ, Silva JC, Borges JP. Produção de fibras de hidroxiapatita por electrofiação. Ciência & Tecnologia dos Materiais. 2010;22:57–64.Google Scholar