Differentiation of human adipose-derived stem cells seeded on mineralized electrospun co-axial poly(ε-caprolactone) (PCL)/gelatin nanofibers

  • Ildeu H. L. Pereira
  • Eliane Ayres
  • Luc Averous
  • Guy Schlatter
  • Anne Hebraud
  • Ana Cláudia Chagas de Paula
  • Pedro Henrique Leroy Viana
  • Alfredo Miranda Goes
  • Rodrigo L. Oréfice
Article

Abstract

Mineralized poly(ε-caprolactone)/gelatin core–shell nanofibers were prepared via co-axial electrospinning and subsequent incubation in biomimetic simulated body fluid containing ten times the calcium and phosphate ion concentrations found in human blood plasma. The deposition of calcium phosphate on the nanofiber surfaces was investigated through scanning electronic microscopy and X-ray diffraction. Energy dispersive spectroscopy results indicated that calcium-deficient hydroxyapatite had grown on the fibers. Fourier transform infrared spectroscopy analysis suggested the presence of hydroxyl-carbonate-apatite. The results of a viability assay (MTT) and alkaline phosphatase activity analysis suggested that these mineralized matrices promote osteogenic differentiation of human adipose-derived stem cells (hASCs) when cultured in an osteogenic medium and have the potential to be used as a scaffold in bone tissue engineering. hASCs cultured in the presence of nanofibers in endothelial differentiation medium showed lower rates of proliferation than cells cultured without the nanofibers. However, endothelial cell markers were detected in cells cultured in the presence of nanofibers in endothelial differentiation medium.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Ildeu H. L. Pereira
    • 1
  • Eliane Ayres
    • 2
  • Luc Averous
    • 3
  • Guy Schlatter
    • 3
  • Anne Hebraud
    • 3
  • Ana Cláudia Chagas de Paula
    • 4
  • Pedro Henrique Leroy Viana
    • 4
  • Alfredo Miranda Goes
    • 4
  • Rodrigo L. Oréfice
    • 1
  1. 1.Department of Metallurgical and Materials EngineeringFederal University of Minas Gerais (UFMG)Belo HorizonteBrazil
  2. 2.Department of Materials, Technologies and ProcessesSchool of Design, Minas Gerais State University (UFMG)Belo HorizonteBrazil
  3. 3.ICPEES-ECPMUMR 7515, Université de StrasbourgStrasbourg Cedex 2France
  4. 4.Department of Biochemistry and ImunologyFederal University of Minas Gerais (UFMG)Belo HorizonteBrazil

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