The inhibition of lamellar hydroxyapatite and lamellar magnetic hydroxyapatite on the migration and adhesion of breast cancer cells

  • Jun Jin
  • Guifu Zuo
  • Guangyao Xiong
  • Honglin Luo
  • Qiuping Li
  • Chunying Ma
  • Deying Li
  • Feng Gu
  • Yongjie Ma
  • Yizao WanEmail author


Hydroxyapatite nanoparticles have been reported to exhibit potent anti-tumor effects in some cancer cells. In our previous study, we have successfully synthesized two types of hydroxyapatite nanoparticles, laminated hydroxyapatite (L-HAp) and laminated magnetic hydroxyapatite (LM-HAp). In this study, we wanted to investigate the effects of L-HAp and LM-HAp with various concentrations on human breast cancer MDA-MB-231 cells. Cell proliferation was assessed with a MTT colorimetric assay. Scratch and adhesion assays were used to detect the effects of these two materials on migration and adhesion. The expressions of integrin β1 and Akt were measured by Western blotting. Our results showed that L-HAp and LM-HAp had little cell cytotoxicity and significantly reduced cell mobility and adhesion. LM-HAp showed greater inhibitor ability on migration and adhesion of MDA-MB-231 cells. Moreover, results from western blotting showed that L-HAp and LM-HAp impacted the phosphorylation of integrin β1, but showed no regular impact on Akt. This study suggests that L-HAp and LM-HAp may be potential anti-tumor and delivery system for breast cancer therapy.


Breast Cancer Cell Hydroxyapatite Fe3O4 Nanoparticles Adhesion Assay Gene Delivery System 
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This work is supported by the National Natural Science Foundation of China (Grants No. 51172158 and 81200663), the Science and Technology Support Program of Tianjin (Grant No. 11ZCKFSY01700) and the Natural Science Foundation of Hebei Province (Grants No. E2014209204).


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Jun Jin
    • 1
  • Guifu Zuo
    • 3
  • Guangyao Xiong
    • 2
  • Honglin Luo
    • 1
  • Qiuping Li
    • 2
  • Chunying Ma
    • 2
  • Deying Li
    • 2
  • Feng Gu
    • 4
  • Yongjie Ma
    • 5
  • Yizao Wan
    • 1
    Email author
  1. 1.Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and EngineeringTianjin UniversityTianjinPeople’s Republic of China
  2. 2.School of Mechanical and Electrical EngineeringEast China Jiaotong UniversityNanchangPeople’s Republic of China
  3. 3.Hebei Provincial Key Laboratory of Inorganic Nonmetallic Materials, College of Materials Science and EngineeringHebei United UniversityTangshanChina
  4. 4.Department of Breast Cancer Pathology, Cancer Institute and HospitalTianjin Medical UniversityTianjinPeople’s Republic of China
  5. 5.Department of Core Laboratory, Cancer Institute and HospitalTianjin Medical UniversityTianjinPeople’s Republic of China

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