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Dielectric study of the adhesion of mesenchymal stem cells from human umbilical cord on a sugarcane biopolymer

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Abstract

It is of current interest the identification of appropriate matrices for growing mesenchymal stem cells (MSC). These cells are able not only to regenerate themselves but also to differentiate into other type of functional cells, and so they have been extensively used in tissue engineering. In this work, we have evaluated the use of electric impedance spectroscopy (EIS) to follow the adhesion of MSC from Wharton’s jelly of the human umbilical cord (hWJMSC) on sugarcane biopolymers (SCB). Impedance spectra of the systems were obtained in the frequency range of 102–105 Hz. An EIS investigation showed that when deposited on a metallic electrode SCB films prevent the passage of electrons between the solution and the metallic interface. The impedance spectra of hWJMSCs adhered on SCB revealed that there is a significant increase in the magnitude of the impedance when compared to that of pure SCB. The corresponding resistance (real part of the impedance) was even higher for the SCB–hWJMSC system than for SCB without cells on their surface, in an indication of an increased blockage to the electron transfers. The resistance charge transfer is extracted by curve-fitting the impedance spectra to an equivalent circuit model. Also, a shift of the phase angle to higher frequencies was obtained for SCB–hWJMSC system as a result from hWJMSC adhesion. Our study demonstrates that EIS is an appropriate method to evaluate the adhesion of MSC. SCB can be considered as a promising biomaterial for tissue engineering.

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Acknowledgments

We dedicated this work to Prof. Oleg V. Krasilnikov (in memoriam). Fragoso would like to thank FACEPE for a graduate scholarship. Andrade and Oliveira are also gratefully for CNPq financial support (Grant 310305/2012-8 and 310361/2012-5, respectively). We thank the Hospital de Ávila (Recife, PE) for providing human umbilical cord samples.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, 50670-901, Brazil

    A. S. Fragoso & M. D. L. Oliveira

  2. Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, PE, 50670-901, Brazil

    M. B. Silva, C. G. Rodrigues & J. F. Branco Junior

  3. Departamento de Física, Universidade Federal Pernambuco, Recife, PE, 50670-901, Brazil

    C. P. de Melo

  4. Departamento de Cirurgia, Universidade Federal de Pernambuco, Recife, PE, 50670-901, Brazil

    J. L. A. Aguiar

  5. Departamento de Histologia e Embriologia, Universidade Federal de Pernambuco, Recife, PE, 50670-901, Brazil

    P. L. de Medeiros

  6. Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, 50670-901, Brazil

    C. A. S. Andrade & M. D. L. Oliveira

Authors
  1. A. S. Fragoso
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  2. M. B. Silva
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  3. C. P. de Melo
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  4. J. L. A. Aguiar
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  5. C. G. Rodrigues
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  6. P. L. de Medeiros
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  7. J. F. Branco Junior
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  8. C. A. S. Andrade
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  9. M. D. L. Oliveira
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Correspondence to M. D. L. Oliveira.

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Fragoso, A.S., Silva, M.B., de Melo, C.P. et al. Dielectric study of the adhesion of mesenchymal stem cells from human umbilical cord on a sugarcane biopolymer. J Mater Sci: Mater Med 25, 229–237 (2014). https://doi.org/10.1007/s10856-013-5056-5

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  • DOI: https://doi.org/10.1007/s10856-013-5056-5

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