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N-containing functional groups induced superior cytocompatible and hemocompatible graphene by NH2 ion implantation

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Abstract

Graphene is functionalized with amine by NH2 ion implantation at room temperature in vacuum. The reaction is featured by nucleophilic substitution of C–O groups by the ammonia radicals. The presence of N-containing functional groups in graphene is identified by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. N element was successfully introduced to graphene, the atomic ratio of N to C rose to 3.12 %. NH2 ion implanted graphene (G-NH2) is a better hydrophilic material than pristine grahene according to the contact angle experiment. Mouse fibroblast cells and human endothelial cells cultured on G-NH2 displayed superior cell-viability, proliferation and stretching over that on pristine graphene. Platelet adhesion, hemolysis and Kinetic-clotting time were measured on G-NH2, showing excellent anticoagulation, with as good hemolysis as pristine graphene.

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Acknowledgements

This study was supported by National Natural Science Foundation of China (51272176, 11075116), National Basic Research Program of China (973 Program, 2012CB933600), and Youth Foundation of Tianjin Normal University (52XQ1204). The Key Project of Tianjin Municipal Natural Science Foundation of China (13JCZDJC33900) supported partly the design and synthesis of graphene and NH2 ion implanted graphene (G-NH2). We appreciate Minsi Li, undergraduate student in School of Chemistry and Materials Science, University of Science and Technology of China, for her kind idea and contribution to synthesis of graphene and NH2 ion implanted graphene.

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Authors and Affiliations

  1. College of Physics and Electronic Information Science, Tianjin Normal University, Tianjin, 300387, China

    Meixian Guo, Xiaoqi Liu, Mengli Zhao & Dejun Li

  2. School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, 230026, China

    Minsi Li

  3. Department of Mechanical & Materials Engineering, University of Western Ontario, London, ON, N5X 4J6, Canada

    Dongsheng Geng & Xueliang Sun

  4. Tianjin Institute of Urological Surgery, Tianjin Medical University, Tianjin, 300070, China

    Hanqing Gu

  5. Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China

    Hanqing Gu

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  1. Meixian Guo
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Correspondence to Dejun Li.

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Guo, M., Li, M., Liu, X. et al. N-containing functional groups induced superior cytocompatible and hemocompatible graphene by NH2 ion implantation. J Mater Sci: Mater Med 24, 2741–2748 (2013). https://doi.org/10.1007/s10856-013-5016-0

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  • DOI: https://doi.org/10.1007/s10856-013-5016-0

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