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Journal of Materials Science: Materials in Medicine

, Volume 24, Issue 11, pp 2577–2587 | Cite as

Emulsion electrospun nanofibers as substrates for cardiomyogenic differentiation of mesenchymal stem cells

  • Lingling Tian
  • Molamma P. PrabhakaranEmail author
  • Xin DingEmail author
  • Dan Kai
  • Seeram Ramakrishna
Article

Abstract

The potential of cardiomyogenic differentiation of human mesenchymal stem cells (hMSCs) on emulsion electrospun scaffold containing poly(l-lactic acid)-co-poly-(ε-caprolactone), gelatin and vascular endothelial growth factor (PLCL/GV) was investigated in this study. The characterizations of the scaffold were carried out using scanning electron microscope (SEM), transmission electron microscope, water contact angle and porometer. The proliferation of hMSCs showed that 73.4 % higher cell proliferation on PLCL/GV scaffolds than that on PLCL scaffold after 20 days of cell culture. Results of 5-chloromethylfluorescein diacetate staining and SEM morphology analysis indicated that hMSCs differentiated on PLCL/GV scaffolds showed irregular morphology of cardiomyocyte phenotype compared to the typical long and thin hMSC phenotype. Immunostaining results showed the expression of alpha actinin and myosin heavy chain. Our studies identified emulsion electrospinning as a method for fabrication of core–shell fibers suitable for the differentiation of stem cells to cardiac cells, with potential application in cardiac regeneration.

Keywords

Vascular Endothelial Growth Factor Water Contact Angle Electrospun Nanofibers Vascular Endothelial Growth Factor Protein Nanofibrous Scaffold 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research is supported by the Singapore National Research Foundation under CREATE programme: The Regenerative Medicine Initiative in Cardiac Restoration Therapy (NRF-Technion R-398-001-065-592). Ms Lingling Tian would also like to acknowledge the China Scholarship Council for granting a scholarship that enabled her to pursue this work at NUS, Singapore.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Key Laboratory of Textile Science & TechnologyMinistry of Education of China, Donghua UniversityShanghaiChina
  2. 2.College of TextilesDonghua UniversityShanghaiChina
  3. 3.Center for Nanofibers & Nanotechnology, E3-05-14, Nanoscience and Nanotechnology Initiative, Faculty of EngineeringNational University of SingaporeSingaporeSingapore
  4. 4.NUS Graduate School for Integrative Sciences and EngineeringNational University of SingaporeSingaporeSingapore

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