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Effects of porous beta-tricalcium phosphate-based ceramics used as an E. coli-derived rhBMP-2 carrier for bone regeneration

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Abstract

Recombinant human bone morphogenetic protein-2 (rhBMP-2) requires carriers for clinical effectiveness. In this study, whether porous beta-tricalcium phosphate (β-TCP)-based ceramics are ideal carriers for rhBMP-2 was investigated. Hydroxyapatite (HA), β-TCP, TCP/HA (80 %/20 %), HA with rhBMP-2, TCP with rhBMP-2, and TCP/HA (80 %/20 %) with rhBMP-2 were manufactured by a sponge method with a pore size of 300 μm or more and macro-porosity of 83 %. The alkaline phosphatase (ALP) activity and ALP expression of the cells with 100 % β-TCP granules were more increased than the those of cells with 100 % HA and TCP/HA (80 %/20 %) at the baseline or when treated with 15 ng/ml of rhBMP-2. In an SD rat calvarial defect model, new bone formation was evidently shown in the TCP 100 %-rhBMP-2 and TCP/HA (80 %/20 %)-rhBMP-2 groups, showing that the most affected area was filled with newly-formed bone, that the percent bone volume and trabecular number were larger when compared to the groups without rhBMP-2 treatment at both 4 and 8 weeks after surgery using micro-CT and histology. Porous TCP-based ceramic granules enhanced the osteoblastic differentiation in the hMSC system when treated with 15 ng/ml of rhBMP-2 and accelerated bone-healing by trabecular number in a rat calvarial defect model. Thus, in this study it was proposed that TCP-based ceramics might be useful carriers of rhBMP-2.

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Acknowledgments

This study was supported by a Grant-in-Aid from the Korean Ministry of Knowledge Economy/KEIT (No. 10033623).

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Authors and Affiliations

  1. Department of Orthopedic Surgery, College of Medicine, Seoul National University, SMG-SNU Boramae Medical Center, 425 Shindaebang-2-Dong, Seoul, 156-707, Korea

    Jae Hyup Lee, Hae-Ri Baek, Kyung Mee Lee & Hyun-Kyung Lee

  2. Institute of Medical and Biological Engineering, Seoul National University, Medical Research Center, Seoul, 110-799, Korea

    Jae Hyup Lee, Hae-Ri Baek & Hyun-Kyung Lee

  3. Research Center, Bioalpha, Sung-Nam, 462-120, Korea

    Mi Young Ryu, Jun-Hyuk Seo & Hyun-Seung Ryu

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  1. Jae Hyup Lee
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  2. Mi Young Ryu
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Correspondence to Jae Hyup Lee.

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Lee, J.H., Ryu, M.Y., Baek, HR. et al. Effects of porous beta-tricalcium phosphate-based ceramics used as an E. coli-derived rhBMP-2 carrier for bone regeneration. J Mater Sci: Mater Med 24, 2117–2127 (2013). https://doi.org/10.1007/s10856-013-4967-5

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  • DOI: https://doi.org/10.1007/s10856-013-4967-5

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