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Synthesis of poly-(3-hydroxybutyrate-co-12 mol % 3-hydroxyvalerate) by Bacillus cereus FB11: its characterization and application as a drug carrier

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Abstract

The synthesis of microbial polyhydroxyalkanoate is investigated in this work for it potential application as drug carrier for cancer therapy. The bacterial isolate Bacillus cereus FB11 has synthesized poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer under nutrient stress conditions using glucose as a sole carbon source. The FTIR spectrum of the purified copolymer showed the characteristic absorption bands at 1,719, 1,260 and 2,931 cm−1 attributing to C=O, C–O stretching and C–H vibrations, respectively. The result of 1H-NMR confirmed that it was composed of 88 mol % of 3-hydroxybutyrate and 12 mol % of 3-hydroxyvalerate monomeric subunits. The nanoparticles were fabricated from copolymer and used as a carrier for anticancer drug ellipticine. The in vitro drug release studies showed that % inhibition of A549 cancer cell line receiving ellipticine loaded poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticles was two-fold higher in comparison to ellipticine alone. This drug delivery system offers exciting possibilities for cancer therapy by increasing the bioavailability of anti-neoplastic drug to the tumor site.

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Acknowledgments

We thank Higher Education Commission (HEC) Pakistan for providing the financial assistance to complete this research work. We also thank to members of Prof. P. Chen research group especially the Lab Manager, Tatiana Sheinin, University of Waterloo, Canada for their helpful guidance. We are deeply indebted to Dr. Ihsan-ul-Haq and Dr. M. Imran, Quaid-i-Azam University (QAU), Pakistan, for providing their help in the data analysis in this work.

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  1. Farha Masood

    Present address: Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan

Authors and Affiliations

  1. Department of Microbiology, Quaid-i-Azam University (QAU), Islamabad, Pakistan

    Farha Masood, Fariha Hasan & Abdul Hameed

  2. Department of Chemical Engineering, University of Waterloo (UW), Waterloo, Canada

    P. Chen

  3. Department of Metallurgy and Material Engineering, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan

    Tariq Yasin

  4. Department of Biotechnology and Bioinformatics, International Islamic University (IIU), Islamabad, Pakistan

    Bashir Ahmad

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  1. Farha Masood
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Correspondence to Farha Masood.

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Masood, F., Chen, P., Yasin, T. et al. Synthesis of poly-(3-hydroxybutyrate-co-12 mol % 3-hydroxyvalerate) by Bacillus cereus FB11: its characterization and application as a drug carrier. J Mater Sci: Mater Med 24, 1927–1937 (2013). https://doi.org/10.1007/s10856-013-4946-x

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