Abstract
A high-voltage (10 kV) electrostatic antisolvent process was used to prepare methotrexate (MTX)-loaded, large, highly-porous poly-l-lactide (PLLA) microspheres. MTX solution in dimethyl sulfoxide (DMSO) and PLLA solution in dichloromethane (DCM) were homogeneously mixed, and then ammonium bicarbonate (AB) aqueous solution was added. The mixed solution was emulsified by ultrasonication with Pluronic F127 (PF127) as an emulsion stabilizer. The emulsion was electrosprayed by the specific high-voltage apparatus and dropped into a 100 mL of ethanol, which acted as an antisolvent for the solute and extracted DMSO and DCM, causing the co-precipitation of PLLA and MTX, thus forming microspheres with AB aqueous micro-droplets uniformly inlaid. The obtained MTX–PLLA microspheres were subsequently lyophilized to obtain large, highly-porous MTX–PLLA microspheres, which exhibited an identifiable spherical shape and a rough surface furnished with open pores, with a mean particle size of 25.0 μm, mass median aerodynamic diameter of 3.1 ± 0.2 μm, fine-particle fraction of 57.1 ± 1.6 %, and porosity of 81.8 %; furthermore, they offered a sustained release of MTX. X-ray diffraction and Fourier transform-infrared spectra revealed that no crystallinity or alteration of chemical structure occurred during the high-voltage electrostatic antisolvent process, which in this study was proved to have great potential for preparing highly-porous drug-loaded polymer microspheres for use in pulmonary drug delivery.
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Meenach SA, Kim YJ, Kauffman KJ, Kanthamneni N, Bachelder EM, Ainslie KM. Synthesis, optimization, and characterization of camptothecin-loaded acetalated dextran porous microparticles for pulmonary delivery. Mol Pharm. 2012;9:290–8.
Arnold MM, Gonnan EM, Schieber LJ, Munson EJ, Berkland C. NanoCipro encapsulation in monodisperse large porous PLGA microparticles. J Control Release. 2007;121:100–9.
Ungaro F, Giovino C, Coletta C, Sorrentino R, Miro A, Quaglia F. Engineering gas-foamed large porous particles for efficient local delivery of macromolecules to the lung. Eur J Pharm Sci. 2010;41:60–70.
Gupta V, Ahsan F. Influence of PEI as a core modifying agent on PLGA microspheres of PGE1, a pulmonary selective vasodilator. Int J Pharm. 2011;413:51–62.
Kwona MJ, Baea JH, Kima JJ, Nab K, Lee ES. Long acting porous microparticle for pulmonary protein delivery. Int J Pharm. 2007;333:5–9.
Kirch J, Guenther M, Doshi N, Schaefer UF, Schneider M, Mitragotri S, Lehr CM. Mucociliary clearance of micro- and nanoparticles is independent of size, shape and charge-an ex vivo and in silico approach. J Control Release. 2012;159:128–34.
Hadinoto K, Zhu K, Tan RBH. Drug release study of large hollow nanoparticulate aggregates carrier particles for pulmonary delivery. Int J Pharm. 2007;341:195–206.
Beck-Broichsitter M, Merkel OM, Kissel T. Controlled pulmonary drug and gene delivery using polymeric nano-carriers. J Control Release. 2012;161:214–24.
Oh YJ, Lee J, Seo JY, Rhim T, Kim SH, Yoon HJ, Lee KY. Preparation of budesonide-loaded porous PLGA microparticles and their therapeutic efficacy in a murine asthma model. J Control Release. 2011;150:56–62.
Beck-Broichsitter M, Schweiger C, Schmehl T, Gessler T, Seeger W, Kissel T. Characterization of novel spray-dried polymeric particles for controlled pulmonary drug delivery. J Control Release. 2012;158:329–35.
Lee J, Oh YJ, Lee SK, Lee KY. Facile control of porous structures of polymer microspheres using an osmotic agent for pulmonary delivery. J Control Release. 2010;146:61–7.
Yang Y, Bajaj N, Xu P, Ohn K, Tsifansky MD, Yeo Y. Development of highly porous large PLGA microparticles for pulmonary drug delivery. Biomaterials. 2009;30:1947–53.
Kim HK, Chung HJ, Park TG. Biodegradable polymeric microspheres with “open/closed” pores for sustained release of human growth hormone. J Control Release. 2006;112:167–74.
Almeria B, Deng W, Fahmy TM, Gomez A. Controlling the morphology of electrospray-generated PLGA microparticles for drug delivery. J Colloid Interface Sci. 2010;343:125–33.
Xu Y, Hanna MA. Electrospray encapsulation of water-soluble protein with polylactide—effects of formulations on morphology, encapsulation efficiency and release profile of particles. Int J Pharm. 2006;320:30–6.
Zhang W, He X. Encapsulation of living cells in small (approximately 100 μm) alginate microcapsules by electrostatic spraying: a parametric study. J Biomech Eng. 2009;131:074515.
Zhou Y, Sun T, Chan M, Zhang J, Han ZY, Wang XW, Toh Y, Chen JP, Yu H. Scalable encapsulation of hepatocytes by electrostatic spraying. J Biotechnol. 2005;117:99–109.
Steckel H, Brandes HG. A novel spray-drying technique to produce low density particles for pulmonary delivery. Int J Pharm. 2004;278:187–95.
Xie JW, Wang CH. Encapsulation of proteins in biodegradable polymeric microparticles using electrospray in the Taylor Cone-Jet mode. Biotechnol Bioeng. 2007;97:1278–90.
Reyderman L, Stavchansky S. Electrostatic spraying and its use in drug delivery-cholesterol microspheres. Int J Pharm. 1995;124:75–85.
Berkland C, Pack DW, Kim K. Controlling surface nano-structure using flow-limited field-injection electrostatic spraying (FFESS) of poly(d, l-lactide-co-glycolide). Biomaterials. 2004;25:5649–58.
Chow AHL, Tong HHY, Chattopadhyay P, Shekunov BY. Particle engineering for pulmonary drug delivery. Pharm Res. 2007;24:411–37.
Giovagnoli S, Blasi P, Schoubben A, Rossi C, Ricci M. Preparation of large porous biodegradable microspheres by using a simple double-emulsion method for capreomycin sulfate pulmonary delivery. Int J Pharm. 2007;333:103–11.
Wan F, Møller EH, Yang M, Jørgensen L. Formulation technologies to overcome unfavorable properties of peptides and proteins for pulmonary delivery. Drug Discov Today Technol. 2012;9:e141–6.
Huang X, Brazel CS. On the importance and mechanisms of burst release in matrix-controlled drug delivery systems. J Control Release. 2001;73:121–36.
Duncan G, Jess TJ, Mohamed F, Price NC, Kelly SM, van der Walle CF. The influence of protein solubilisation, conformation and size on the burst release from poly(lactide-co-glycolide) microspheres. J Control Release. 2005;110:34–48.
Wolinsky JB, Colson YL, Grinstaff MW. Local drug delivery strategies for cancer treatment: gels, nanoparticles, polymeric films, rods, and wafers. J Control Release. 2012;159:14–26.
Siepmann F, Siepmann J, Walther M, MacRae RJ, Bodmeier R. Polymer blends for controlled elease coatings. J Control Release. 2008;125:1–15.
Borgquist P, Korner A, Piculell L, Larsson A, Axelsson A. A model for the drug release from a polymer matrix tablet—effects of swelling and dissolution. J Control Release. 2006;113:216–25.
Shrewsbury SB, Bosco AP, Uster PS. Pharmacokinetics of a novel submicron budesonide dispersion for nebulized delivery in asthma. Int J Pharm. 2009;365:12–7.
Ibrahim BM, Park S, Han B, Yeo Y. A strategy to deliver genes to cystic fibrosis lungs: a battle with environment. J Control Release. 2011;155:289–95.
Anabousi S, Bakowsky U, Schneider M, Huwer H, Lehr CM, Ehrhardt C. In vitro assessment of transferrin-conjugated liposomes as drug delivery systems for inhalation therapy of lung cancer. Eur J Pharm Sci. 2006;29:367–74.
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Financial supports from Natural Science Foundation of Fujian Province (2010J05027 and 2011J01223) and National Natural Science Foundation of China (51103049, 81171471 and 31170939) are gratefully acknowledged.
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Chen, AZ., Yang, YM., Wang, SB. et al. Preparation of methotrexate-loaded, large, highly-porous PLLA microspheres by a high-voltage electrostatic antisolvent process. J Mater Sci: Mater Med 24, 1917–1925 (2013). https://doi.org/10.1007/s10856-013-4942-1
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DOI: https://doi.org/10.1007/s10856-013-4942-1