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Characterization and biocompatibility studies of new degradable poly(urea)urethanes prepared with arginine, glycine or aspartic acid as chain extenders

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Abstract

Polyurethanes are very often used in the cardiovascular field due to their tunable physicochemical properties and acceptable hemocompatibility although they suffer from poor endothelialization. With this in mind, we proposed the synthesis of a family of degradable segmented poly(urea)urethanes (SPUUs) using amino acids (l-arginine, glycine and l-aspartic acid) as chain extenders. These polymers degraded slowly in PBS (pH 7.4) after 24 weeks via a gradual decrease in molecular weight. In contrast, accelerated degradation showed higher mass loss under acidic, alkaline and oxidative media. MTT tests on polyurethanes with l-arginine as chain extenders showed no adverse effect on the metabolism of human umbilical vein endothelial cells (HUVECs) indicating the leachables did not provoke any toxic responses. In addition, SPUUs containing l-arginine promoted higher levels of HUVECs adhesion, spreading and viability after 7 days compared to the commonly used Tecoflex® polyurethane. The biodegradability and HUVEC proliferation on l-arginine-based SPUUs suggests that they can be used in the design of vascular grafts for tissue engineering.

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Acknowledgments

This work was supported by CONACYT (México) Grants 79371, 123913 and FOMIX 108160. The authors also acknowledge the Ministère des relations internationales du Québec (JOOO.0463) for funding the exchange program between Mexico and Québec. We also thank Dr. Frederick Morin from McGill NMR facility and D. H. Aguilar for their technical support.

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Authors and Affiliations

  1. Centro de Investigación Científica de Yucatán A. C., Calle 43 # 130 Col. Chuburná de Hidalgo, C.P.97200, Mérida, Yucatán, Mexico

    L. H. Chan-Chan, R. F. Vargas-Coronado, J. M. Cervantes-Uc & J. V. Cauich-Rodriguez

  2. Department of Biomedical Engineering, Mc Gill University, Duff Medical Building, 3775 University Street, Montreal, Quebec, H3A 2B4, Canada

    C. Tkaczyk & M. Tabrizian

  3. Faculty of Dentistry, Mc Gill University, 3480 University Street, Montreal, Quebec, H3A 2B2, Canada

    M. Tabrizian

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  1. L. H. Chan-Chan
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  2. C. Tkaczyk
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  3. R. F. Vargas-Coronado
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  4. J. M. Cervantes-Uc
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  6. J. V. Cauich-Rodriguez
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Correspondence to J. V. Cauich-Rodriguez.

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Chan-Chan LH and Tkaczyk C equally contributed to this study.

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Chan-Chan, L.H., Tkaczyk, C., Vargas-Coronado, R.F. et al. Characterization and biocompatibility studies of new degradable poly(urea)urethanes prepared with arginine, glycine or aspartic acid as chain extenders. J Mater Sci: Mater Med 24, 1733–1744 (2013). https://doi.org/10.1007/s10856-013-4931-4

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  • DOI: https://doi.org/10.1007/s10856-013-4931-4

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