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Evaluation of early stage human bone marrow stromal proliferation, cell migration and osteogenic differentiation on μ-MIM structured stainless steel surfaces

  • Malak Bitar
  • Fausta Benini
  • Claudia Brose
  • Vera Friederici
  • Philipp Imgrund
  • Arie BruininkEmail author
Article

Abstract

It is well established that surface topography greatly affect cell—surface interactions. In a recent study we showed that microstructured stainless steel surfaces characterized by the presence of defined hexagonally arranged hemisphere-like structures significantly affected cell architecture (shape and focal adhesion size) of primary human bone mesenchymal stromal cells. This study aimed at further investigating the influence these microstructures (microcline protruding hemispheres) on critical aspects of cell behaviour namely; proliferation, migration and osteogenic differentiation. As with previously reported data, we used primary human bone mesenchymal stromal cells to investigate such effects at an early stage in vitro. Cells of different patients were utilised for cell migration studies. Our data showed that an increase in cell proliferation was exhibited as a function of surface topography (hemispheres). Cell migration velocity also varied as a function of surface topography on patient specific basis and seems to relate to the differentiated state of the seeded cell population (as demonstrated by bALP positivity). Osteogenic differentiation, however, did not exhibit significant variations (both up and down-regulation) as a function of both surface topography and time in culture.

Keywords

Osteogenic Differentiation Migration Velocity Human Bone Marrow Stromal Cell Cell Trajectory Metal Injection Moulding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors acknowledge the generous support of the Volkswagen Foundation (contract nr. 182–296) in Germany.We also thank Kantonsspital St. Gallen (CH) for providing the human tissue samples.

Supplementary material

10856_2013_4876_MOESM1_ESM.docx (44 kb)
Supplementary material 1 (DOCX 43 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Malak Bitar
    • 1
    • 3
  • Fausta Benini
    • 1
  • Claudia Brose
    • 1
  • Vera Friederici
    • 2
  • Philipp Imgrund
    • 2
  • Arie Bruinink
    • 1
    Email author
  1. 1.Materials-Biology Interactions LabEMPASt. GallenSwitzerland
  2. 2.Fraunhofer Institute for Manufacturing and Advanced Materials (IFAM)BremenGermany
  3. 3.Technical Research and DevelopmentNovartis Pharma AGBaselSwitzerland

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