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Promotion of initial cell adhesion on trisuccinimidyl citrate-modified nickel-free high-nitrogen stainless steel

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Abstract

The surface of nickel-free high-nitrogen stainless steel (HNS) was modified with a citric acid-based cross-linker, trisuccinimidyl citrate (TSC), to promote initial cell adhesion in external skeletal fixation pins. The remaining active ester groups on TSC-immobilized HNS reacted with the amino groups of serum proteins. The immobilized serum proteins formed cell recognition sites to promote the initial cell adhesion immediately after cell seeding. The amount of fibronectin, which is a typical cell adhesion protein, immobilized on the TSC-immobilized HNS surface was threefold greater than on the original HNS after only 15 min. The fibroblastic cell culture experiments showed that the initial cell adhesion was significantly enhanced on the TSC-immobilized HNS compared with the original HNS at 3 h. Furthermore, the cell adhesion activity of the TSC-immobilized HNS continued to promote cell proliferation even at 7 days. Therefore, TSC-immobilized HNS may enable the rapid integration of soft tissues through its reaction with the patient’s serum proteins and extracellular proteins around the surgical site.

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Acknowledgments

We thank Ms. M. Ueno and Ms. T. Ishizuka of Biomaterials Unit, Nano-Life Field, National Institute for Materials Science of Japan, for technical support. This work was supported financially in part by the Japan Society for the Promotion of Science (JSPS) through the Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program), initiated by the Council for Science and Technology Policy (CSTP), MANA, and the World Premier International Research Center (WPI) Initiative on Materials Nanoarchitectonics, MEXT, Japan.

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Authors and Affiliations

  1. Graduate School of Pure and Applied Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan

    Makoto Sasaki & Tetsushi Taguchi

  2. Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan

    Makoto Sasaki, Motoki Inoue, Yasuyuki Katada & Tetsushi Taguchi

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  1. Makoto Sasaki
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  2. Motoki Inoue
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  3. Yasuyuki Katada
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  4. Tetsushi Taguchi
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Correspondence to Tetsushi Taguchi.

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Sasaki, M., Inoue, M., Katada, Y. et al. Promotion of initial cell adhesion on trisuccinimidyl citrate-modified nickel-free high-nitrogen stainless steel. J Mater Sci: Mater Med 24, 951–958 (2013). https://doi.org/10.1007/s10856-012-4845-6

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  • DOI: https://doi.org/10.1007/s10856-012-4845-6

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