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Layered double hydroxides as efficient drug delivery system of ciprofloxacin in the middle ear: an animal study in rabbits

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Abstract

Chronic otitis media is a common disease often accompanied by recurrent bacterial infections. These may lead to the destruction of the middle ear bones such that prostheses have to be implanted to restore sound transmission. Surface coatings with layered double hydroxides (LDHs) are evaluated here as a possibility for drug delivery systems with convenient advantages such as low cytotoxicity and easy synthesis. Male New Zealand White rabbits were implanted with Bioverit® II middle ear prostheses coated with the LDH Mg4Al2(OH)12(SO4)2·6H2O impregnated with ciprofloxacin. 12 (group 1) were directly infected with Pseudomonas aeruginosa and another 12 (group 2) 1 week after the implantation. Clinical outcome, blood counts, histological analyses and microbiological examination showed an excellent antimicrobial activity for group 1, whereas this effect was attenuated in animals where infection was performed 1 week after implantation. This is the first study to demonstrate an efficient drug delivery system with an LDH coating on prostheses in the middle ear.

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Acknowledgments

This work was supported by the German Research Foundation (DFG, Collaborative Research Centre SFB 599, work packages D1 and DR1). The authors would like to thank Susanne Häussler from Helmholtz Centre for Infection Research (Braunschweig, Germany) for the kind provision of the Ps. aeruginosa strain “PAO1”. Moreover, Muhammad Badar was supported by the Higher Education Commission (HEC) and German Academic Exchange Service (DAAD).

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Authors and Affiliations

  1. ENT Department, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany

    Daniela Hesse, Martin Stieve & Nils Kristian Prenzler

  2. Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany

    Muhammad Badar & Peter Paul Müller

  3. Institute of Laboratory Animal Science and Central Animal Facility, Medical University of Hannover, Carl-Neuberg-Str. 1, 30625, Hannover, Germany

    André Bleich, Anna Smoczek & Silke Glage

  4. Institute for Inorganic Chemistry, Leibnitz University of Hannover, Callinstr. 9, 30167, Hannover, Germany

    Marc Kieke & Peter Behrens

  5. Institute of Zoology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559, Hannover, Germany

    Karl-Heinz Esser

Authors
  1. Daniela Hesse
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  2. Muhammad Badar
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  3. André Bleich
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  4. Anna Smoczek
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  5. Silke Glage
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  6. Marc Kieke
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  7. Peter Behrens
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  8. Peter Paul Müller
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  9. Karl-Heinz Esser
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  10. Martin Stieve
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  11. Nils Kristian Prenzler
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Corresponding author

Correspondence to Nils Kristian Prenzler.

Additional information

M. Stieve and N. K. Prenzler contributed equaly to this study.

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Hesse, D., Badar, M., Bleich, A. et al. Layered double hydroxides as efficient drug delivery system of ciprofloxacin in the middle ear: an animal study in rabbits. J Mater Sci: Mater Med 24, 129–136 (2013). https://doi.org/10.1007/s10856-012-4769-1

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  • DOI: https://doi.org/10.1007/s10856-012-4769-1

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