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Radiolabeled gelatin type B analogues can be used for non-invasive visualisation and quantification of protein coatings on 3D porous implants

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Abstract

This study covers the quantification of the covalent attachment of gelatin type B (GelB) and the subsequent adsorption of Fibronectin (Fn) on poly-ε-caprolactone (PCL) surfaces, functionalised with 2-aminoethyl methacrylate (AEMA) by means of post-plasma UV-irradiation grafting. As typical surface characterisation tools do not allow quantification of deposited amounts of GelB or Fn, radiolabeled analogues were used for direct measurement of the amount of immobilized material. Bolton-Hunter GelB (BHG) and Fn were radioiodinated with 131I and 125I respectively and S-Hynic GelB (SHG) was labeled with 99mTc. Immobilisation of 131I-BHG or 99mTc-SHG on both PCL and PCL-AEMA scaffolds was performed in analogy with earlier work. SPECT images on scaffolds coated with 99mTc-SHG conjugates were acquired on a U-SPECT II camera. There was a clear difference in the amount of deposited 131I-BHG between blanco and AEMA-grafted PCL on 2D samples. No significant differences in immobilization behaviour were observed between 99mTc-SHG and 131I-BHG. Subsequent immobilisation of Fn was successful and depended on the amounts of deposited GelB. SPECT imaging on cylindrical 3D scaffolds confirmed these findings and showed that the amount of immobilized 99mTc-SHG was depth dependant. The architecture of the scaffolds strongly influences the distribution of GelB within these structures. Furthermore, there is a clear difference in the homogeneity of the protein coating when different GelB immobilization protocols were applied. This study shows that radiolabeled compounds are a rapid and accurate tool in the quantitative and qualitative evaluation of the biofunctionalisation of AEMA grafted PCL scaffolds.

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Authors and Affiliations

  1. Laboratory for Radiopharmacy, Gent University, Harelbekestraat 72, 9000, Ghent, Belgium

    Ken Kersemans & Filip De Vos

  2. Polymer Chemistry and Biomaterials Research Group, Gent University, Ghent, Belgium

    Tim Desmet & Peter Dubruel

  3. Department IBITech-MEDISIP-INFINITY-GROUP-ID Consortium, Gent University, Ghent, Belgium

    Christian Vanhove

Authors
  1. Ken Kersemans
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  2. Tim Desmet
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  3. Christian Vanhove
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  4. Peter Dubruel
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  5. Filip De Vos
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Correspondence to Ken Kersemans.

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Ken Kersemans, Tim Desmet contributed equally to this publication.

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Kersemans, K., Desmet, T., Vanhove, C. et al. Radiolabeled gelatin type B analogues can be used for non-invasive visualisation and quantification of protein coatings on 3D porous implants. J Mater Sci: Mater Med 23, 1961–1969 (2012). https://doi.org/10.1007/s10856-012-4668-5

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  • DOI: https://doi.org/10.1007/s10856-012-4668-5

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