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Electrospinning collagen and hyaluronic acid nanofiber meshes

Abstract

Collagen and hyaluronic acid (HA) are main components of the extracellular matrix and have been utilized in electrospinning; a technique that creates nanosized fibers for tissue scaffolds. A collagen/HA polymer solution was electrospun into a scaffold material for osteoporosis patients who have reduced bone strength. To synthesize nanofibers, a high voltage was applied to the polymer solution to draw out nanofibers that were collected on a ground plate as a uniform mesh. The meshes were then crosslinked to render them insoluble and conjugated with gold nanoparticles to promote biocompatibility. Characterization of the mesh was performed using scanning electron microscope, electron dispersive spectroscopy and fourier transform infrared spectroscopy. A WST-1 assay determined the potential biocompatibility. The results show that collagen/HA scaffolds were developed that were insoluble in aqueous solutions and promoted cellular attachment that could be used as a tissue engineered scaffold to promote cell growth.

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Acknowledgments

This research was supported in part by the University of Missouri and the BICAM (Biomaterials Innovation, Characterization, and Analysis of Missouri) laboratory. The authors would like to thank Dr. Mohamed Khalid, Assistant Professor of Orthopedic Surgery at the University of Missouri, for developing the concept for this project, and the University of Missouri Electron Microscopy Core for assistance with the imaging in this study.

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Correspondence to Sheila A. Grant.

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Fischer, R.L., McCoy, M.G. & Grant, S.A. Electrospinning collagen and hyaluronic acid nanofiber meshes. J Mater Sci: Mater Med 23, 1645–1654 (2012). https://doi.org/10.1007/s10856-012-4641-3

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Keywords

  • Hyaluronic Acid
  • Mesh Type
  • Fiber Mesh
  • Ground Plate
  • Nanofiber Scaffold