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A simple drug anchoring microfiber scaffold for chondrocyte seeding and proliferation

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Abstract

The structural properties of microfiber meshes made from poly(2-hydroxyethyl methacrylate) (PHEMA) were found to significantly depend on the chemical composition and subsequent cross-linking and nebulization processes. PHEMA microfibres showed promise as scaffolds for chondrocyte seeding and proliferation. Moreover, the peak liposome adhesion to PHEMA microfiber scaffolds observed in our study resulted in the development of a simple drug anchoring system. Attached foetal bovine serum-loaded liposomes significantly improved both chondrocyte adhesion and proliferation. In conclusion, fibrous scaffolds from PHEMA are promising materials for tissue engineering and, in combination with liposomes, can serve as a simple drug delivery tool.

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Acknowledgments

The authors would like to acknowledge J. Farberova from the Technical University of Liberec for measurements of fiber diameters and Sam Norris for proof reading of this manuscript. Supported by the Academy of Sciences of the Czech Republic (institutional research plans AV0Z50390703 and AV0Z50390512), the Ministry of Education, Youth and Sports of the Czech Republic (research programs NPV II 2B06130 and 1M0510, research project CARSILA number ME10145), the Grant Agency of the Academy of Sciences Grant No. IAA500390702 and by the Czech Science Foundation Grant No. GA202/09/1151, IGA MZCR, No. NT12156, and the Grant Agency of the Charles University Grant No. 122508.

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Authors and Affiliations

  1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Michala Rampichová, Eva Filová, Andrea Míčková, Matěj Buzgo & Evžen Amler

  2. Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, V Úvalu 84, 150 06, Prague 5, Czech Republic

    Michala Rampichová, Andrea Míčková, Matěj Buzgo & Evžen Amler

  3. Department of Nonwoven Textiles, Faculty of Textile Engineering, Technical University of Liberec, Liberec, Czech Republic

    Lenka Martinová, Eva Košťáková & David Lukáš

  4. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v. v. i., Prague 6, Czech Republic

    Jiří Michálek & Martin Přádný

  5. Center for Cell Therapy and Tissue Repair, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

    Jiří Michálek & Martin Přádný

  6. Department of Surgery and Orthopedics, Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic

    Alois Nečas

  7. Faculty of Biomedical Engineering, Czech Technical University, Prague, Czech Republic

    Evžen Amler

Authors
  1. Michala Rampichová
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  2. Lenka Martinová
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  3. Eva Košťáková
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  4. Eva Filová
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  5. Andrea Míčková
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  6. Matěj Buzgo
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  7. Jiří Michálek
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  8. Martin Přádný
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  9. Alois Nečas
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  10. David Lukáš
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  11. Evžen Amler
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Corresponding author

Correspondence to Evžen Amler.

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Rampichová, M., Martinová, L., Košťáková, E. et al. A simple drug anchoring microfiber scaffold for chondrocyte seeding and proliferation. J Mater Sci: Mater Med 23, 555–563 (2012). https://doi.org/10.1007/s10856-011-4518-x

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  • DOI: https://doi.org/10.1007/s10856-011-4518-x

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