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Directly created electrostatic micro-domains on hydroxyapatite: probing with a Kelvin Force probe and a protein

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Abstract

Micro-domains of modified surface potential (SP) were created on hydroxyapatite films by direct patterning by mid-energy focused electron beam, typically available as a microprobe of Scanning Electron Microscopes. The SP distribution of these patterns has been studied on sub-micrometer scale by the Kelvin Probe Force Microscopy method as well as lysozyme adsorption. Since the lysozyme is positively charged at physiological pH, it allows us to track positively and negatively charged areas of the SP patterns. Distribution of the adsorbed proteins over the domains was in good agreement with the observed SP patterns.

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References

  1. Zhang PC, Keleshian AM, Sachs F. Voltage-induced membrane movement. Nature. 2001;413:428–31.

    Article  CAS  Google Scholar 

  2. An YH, Friedman RJ. Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. J Biomed Mater Res A. 1998;43:338–48.

    Article  CAS  Google Scholar 

  3. Pashkuleva I, Marques AP, Vaz F, Reis RL. Surface modification of starch based biomaterials by oxygen plasma or UV-irradiation. J Mater Sci: Mater Med. 2010;21:21–32.

    Article  CAS  Google Scholar 

  4. Kato R, Nakamura S, Katayama K, Yamashita K. Electrical polarization of plasma-sprayhydroxyapatite coatings for improvement of osteoconduction of implants. J Biomed Mater Res A. 2005;74A:652–8.

    Article  CAS  Google Scholar 

  5. Cui FZ, Luo ZS. Biomaterials modification by ionbeam processing. Surf Coat Technol. 1999;112:278–85.

    Article  CAS  Google Scholar 

  6. Aronov D, Rosen R, Ron EZ, Rosenman G. Electron-induced surface modification of hydroxyapatite-coated implant. Surf Coat Technol. 2008;202:2093–102.

    Article  CAS  Google Scholar 

  7. Plecenik T, Tofail SAM, Gregor M, et al. Direct creation of microdomains with positive and negative surface potential on hydroxyapatite coatings. Appl Phys Lett. 2011;98:113701.

    Google Scholar 

  8. Lang, SB, Tofail SAM, Gandhi AA, et al. Pyroelectric, piezoelectric, and photoeffects in hydroxyapatite thin films on silicon. Appl Phys Lett. 2011;98:123703.

    Google Scholar 

  9. Arai T, Norde W. The behavior of some model proteins at solid–liquid interfaces 1. Adsorption from single protein solutions. Colloid Surface. 1990;51:1–15.

    Article  CAS  Google Scholar 

  10. Xie Y, Zhou J, Jiang S. Parallel tempering Monte Carlo simulations of lysozyme orientation on charged surfaces. J Chem Phys. 2010;132:065101.

    Article  Google Scholar 

  11. Pasche S, Vörös J, Griesser HJ, Spencer ND, Textor M. Effects of ionic strength and surface charge on protein adsorption at PEGylated surfaces. J Phys Chem B. 2005;109:17545–52.

    Article  CAS  Google Scholar 

  12. Kandori K, Takaragi S-i, Murata K, Ishikawa T. Thermodynamic study of protein adsorption onto synthetic calcium hydroxyapatites. Adsorpt Sci Technol. 2005;23:791–800.

    Article  CAS  Google Scholar 

  13. Kim DT, Blanch HW, Radke CJ. Direct imaging of lysozyme adsorption onto mica by atomic force microscopy. Langmuir. 2002;18:5841–50.

    Article  CAS  Google Scholar 

  14. Barroug J, Lemaitre A, Rouxhet PG. Lysozyme on apatites: a model of protein adsorption controlled by electrostatic interactions. Colloid Surface. 1989;37:339–55.

    Article  Google Scholar 

  15. Kandori K, Mukai M, Fujiwara A, Yasukawa A, Ishikawa T. Adsorption of bovine serum albumin and lysozyme on hydrophobic calcium hydroxyapatites. J Colloid Interf Sci. 1999;212:600–3.

    Article  CAS  Google Scholar 

  16. Aronov D, Rosenman S. Direct low-energy electron beam nanolithography. Surf Sci. 2009;603:2430–3.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This project has been funded with support from the European Commission (EC NMP4-SL-2008-212533—BioElectricSurface). This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein. This work was also supported by the Slovak Research and Development Agency under the contract Nos. DO7RP-007-09 and APVV-0199-10, by the Ministry of Education of the Slovak Republic under Contract Nos VEGA 1/0162/10 and VEGA 1/0605/12 and is also the result of the project implementation: 2622020004 supported by the Research & Development Operational Programme funded by the ERDF.

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Authors and Affiliations

  1. Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, 842 48, Bratislava, Slovakia

    Tomas Plecenik, Maros Gregor, Martin Truchly, Miroslav Zahoran, Peter Kus & Andrej Plecenik

  2. Material and Surface Science Institute, University of Limerick, Limerick, Ireland

    Sylvain Robin, Abbasi Gandhi, Fathima Laffir, Tewfik Soulimane & S. A. M. Tofail

  3. Department of Chemical Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel

    Sidney Lang

  4. Department of Inorganic Chemistry, Faculty of Natural Sciences, Comenius University, 842 15, Bratislava, Slovakia

    Melinda Vargova & Gustav Plesch

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  1. Tomas Plecenik
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  2. Sylvain Robin
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  3. Maros Gregor
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  4. Martin Truchly
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  5. Sidney Lang
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  6. Abbasi Gandhi
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  7. Miroslav Zahoran
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  8. Fathima Laffir
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  9. Tewfik Soulimane
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  10. Melinda Vargova
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  11. Gustav Plesch
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  12. Peter Kus
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  13. Andrej Plecenik
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  14. S. A. M. Tofail
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Correspondence to Tomas Plecenik.

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Plecenik, T., Robin, S., Gregor, M. et al. Directly created electrostatic micro-domains on hydroxyapatite: probing with a Kelvin Force probe and a protein. J Mater Sci: Mater Med 23, 47–50 (2012). https://doi.org/10.1007/s10856-011-4498-x

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  • DOI: https://doi.org/10.1007/s10856-011-4498-x

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