Abstract
Bionanocomposite scaffolds comprised of nanomaterials and the extracellular matrix (ECM) of porcine diaphragm tissue capitalizes on the benefits of utilizing a natural ECM material, while also potentially enhancing physicomechanical properties and biocompatibility through nanomaterials. Gold nanoparticle (AuNP) bionanocomposite scaffolds were subjected to a number of characterization techniques to determine whether the fabrication process negatively impacted the properties of the porcine diaphragm tissue and whether the AuNP improved the properties of the tissue. Tensile testing and differential scanning calorimetry demonstrated that the bionanocomposite possessed improved tensile strength and thermal stability relative to natural tissue. The collagenase assay and Fourier transform infrared spectroscopy additionally confirmed that denaturation of the collagen of the ECM did not occur. The novel bionanocomposite scaffold possessed properties similar to commercially available scaffolds and will be further developed for soft tissue applications such as hernia repair through in vivo studies in an animal model.
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Acknowledgments
The scanning electron microscope utilized in this study was an FEI Quanta FEG 600 FEG acquired under NSF award #ECS-0619607, award #PRM-06-029, and other internal funds from the University of Missouri. This research was supported in part by a National Science Foundation Graduate Research Fellowship, an Internal Project Award from the Department of Surgery at the University of Missouri, and the University of Missouri Food for the twenty-first century (F21C) grant. The authors would also like to thank David Grant, J. Todd Vassalli, and Braden Eliason for their contributions to this study.
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Deeken, C.R., Bachman, S.L., Ramshaw, B.J. et al. Characterization of bionanocomposite scaffolds comprised of mercaptoethylamine-functionalized gold nanoparticles crosslinked to acellular porcine tissue. J Mater Sci: Mater Med 23, 537–546 (2012). https://doi.org/10.1007/s10856-011-4486-1
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DOI: https://doi.org/10.1007/s10856-011-4486-1