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LvBMP-2 gene-modified BMSCs combined with calcium phosphate cement scaffolds for the repair of calvarial defects in rats

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Abstract

The study aims to evaluate the effect of bone marrow stromal cells (BMSCs) expressing bone morphogenic protein-2 (BMP-2) mediated by lentiviral (Lv) gene transduction combined with calcium phosphate cement (CPC) scaffolds for the repair of critical size calvarial defects in rats. BMSCs derived from Fisher 344 rats were transduced with LvBMP-2 or lentivirus encoding enhanced green fluorescent protein (LvEGFP) in vitro. Obvious osteogenic differentiation of BMSCs in the LvBMP-2 group was demonstrated by alkaline phosphatase staining and alizarin red staining. Enzyme-linked immunosorbent assay results show that LvBMP-2 gene expression in vitro can last for at least 8 weeks. Gene-transduced or untransduced BMSCs were seeded onto CPC scaffolds to repair rat calvarial defects with a diameter of 5 mm. Scanning electron microscope analysis indicated that porous CPC scaffolds facilitated initial adhesion and spreading of BMSCs onto its surface. Calvarial defects were successfully repaired with LvBMP-2-transduced BMSCs/CPC constructs 8 weeks postoperatively. The percentage of new bone formation in the LvBMP-2 group was significantly higher than in other control groups. Lentiviral mediated BMP-2 gene therapy together with CPC scaffolds can be used successfully in calvarial repair and bone regeneration.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (Nos. 30772431, 30973342), the Program for New Century Excellent Talents in University (NCET-08-0353), the Science and Technology Commission of Shanghai Municipality (S30206, 0952nm04000, 10430710900, 10dz2211600), Shanghai Rising-star Program (08QH14017), and Shanghai Education Committee (07SG19).

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China

    Chao Zhu, Shaoyi Wang, Jun Zhao, Wenwen Yu & Zhiyuan Zhang

  2. Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China

    Qing Chang

  3. Fourth People’s Hospital, Wuxi, 214000, China

    Qing Chang

  4. School of Stomatology, Tongji University, Shanghai, 200072, China

    Duohong Zou

  5. Oral Bioengineering Lab/Regenerative Medicine Lab, Department of Prosthodontics, Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China

    Wenjie Zhang, Xiuli Zhang & Xinquan Jiang

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  1. Chao Zhu
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  2. Qing Chang
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  3. Duohong Zou
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  4. Wenjie Zhang
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  7. Wenwen Yu
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  8. Xiuli Zhang
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  9. Zhiyuan Zhang
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  10. Xinquan Jiang
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Correspondence to Zhiyuan Zhang or Xinquan Jiang.

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Chao Zhu and Qing Chang are Co-1st authors.

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Zhu, C., Chang, Q., Zou, D. et al. LvBMP-2 gene-modified BMSCs combined with calcium phosphate cement scaffolds for the repair of calvarial defects in rats. J Mater Sci: Mater Med 22, 1965–1973 (2011). https://doi.org/10.1007/s10856-011-4376-6

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  • DOI: https://doi.org/10.1007/s10856-011-4376-6

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