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Electrodeposition of chitosan-glucose oxidase biocomposite onto Pt–Pb nanoparticles modified stainless steel needle electrode for amperometric glucose biosensor

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Abstract

A glucose biosensor was fabricated by electrodepositing chitosan (CS)-glucose oxidase(GOD) biocomposite onto the stainless steel needle electrode (SSN electrode) modified by Pt–Pb nanoparticles (Pt–Pb/SSN electrode). Firstly, Pt–Pb nanoparticles were deposited onto the SSN electrode and then CS-GOD biocomposite was co-electrodeposited onto the Pt–Pb/SSN electrode in a mixed solution containing p-benzoquinone (p-BQ), CS and GOD. The electrochemical results showed that the Pt–Pb nanoparticles can accelerate the electron transfer and improve the effective surface area of the SSN electrode. As a result, the detection range of the proposed biosensor was from 0.03 to 9 mM with a current sensitivity of 0.4485 μA/mM and a response time of 15 s. The Michaelis constant value was calculated to be 4.9837 mM. The cell test results indicated that the electrodes have a low cytotoxicity. This work provided a suitable technology for the fabrication of the needle-type glucose biosensor.

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Acknowledgment

The authors gratefully acknowledge the financial support of the Natural Science Foundation of Tianjin in China (08JC2DJC17900).

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Authors and Affiliations

  1. School of Materials Science and Engineering, Tianjin University, Tianjin, 300072, China

    Meiqing Guo, Haidong Fang, Rui Wang, Zhiqiang Yang & Xinhua Xu

  2. Tianjin Key Laboratory of Composite and Functional Materials, Tianjin, 300072, China

    Xinhua Xu

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  1. Meiqing Guo
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  2. Haidong Fang
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  3. Rui Wang
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  4. Zhiqiang Yang
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  5. Xinhua Xu
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Corresponding author

Correspondence to Xinhua Xu.

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Meiqing Guo and Haidong Fang contributed equally to this work.

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Guo, M., Fang, H., Wang, R. et al. Electrodeposition of chitosan-glucose oxidase biocomposite onto Pt–Pb nanoparticles modified stainless steel needle electrode for amperometric glucose biosensor. J Mater Sci: Mater Med 22, 1985–1992 (2011). https://doi.org/10.1007/s10856-011-4363-y

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  • DOI: https://doi.org/10.1007/s10856-011-4363-y

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