β-Tricalcium-phosphate stimulates the differentiation of dental follicle cells

  • Sandra Viale-Bouroncle
  • Brigitte Bey
  • Torsten E. Reichert
  • Gottfried Schmalz
  • Christian MorsczeckEmail author


The use of dental progenitor cells is a straightforward strategy for regenerative dentistry. For example a cell based therapy with dental follicle cells (DFCs) could be a novel therapeutic strategy for the regeneration of oral tissues in the future. For the regeneration of large bone defects for example dental progenitor cells have to be combined with bone substitutes as scaffolds. This study therefore investigated cell attachment (scanning electron microscopy), cell vitality/proliferation (WST-1 assay) and cell differentiation (under in vitro conditions) of human DFCs on synthetic β-tricalcium phosphate (TCP). DFCs showed considerable cell attachment and proliferation on TCP. Moreover, TCP stimulates osteogenic differentiation in comparison to DFCs with a standard protocol. Here, for example, the osteoblast marker bone sialoprotein (BSP) was highly expressed on TCP, but almost absent in differentiated DFCs without TCP. In conclusion, our study shows that TCP is an excellent scaffold for DFCs for oral tissue regeneration.


Osteogenic Differentiation Bone Substitute Tooth Germ Cell Culture Dish Dental Follicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Conflict of interest



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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Sandra Viale-Bouroncle
    • 1
  • Brigitte Bey
    • 1
  • Torsten E. Reichert
    • 2
  • Gottfried Schmalz
    • 1
  • Christian Morsczeck
    • 1
    Email author
  1. 1.Department of Operative Dentistry and PeriodontologyUniversity of RegensburgRegensburgGermany
  2. 2.Department of Oral and Maxillofacial SurgeryUniversity of RegensburgRegensburgGermany

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