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Development of the novel biotube inserting technique for acceleration of thick-walled autologous tissue-engineered vascular grafts fabrication

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Abstract

To accelerate the fabrication of thick-walled autologous tissue-engineered vascular grafts (TEVGs), a novel biotube inserting technique was developed. After 2 weeks of subcutaneous embedding in rabbits, silicone rods (diameter, 3 mm; length, 35 mm) became encapsulated in connective tissues. Single-layered biotubes were obtained after removing the silicone rods. One silicone rod encapsulated in tissues was inserted into a single-layered biotube to form two layers of autologous tubular tissues. Three layers of autologous tubular tissues were also obtained by applying the same technique. Following a 2-week re-embedding procedure, two layers or three layers of autologous tubular tissues were integrated to form two-layered or three-layered TEVGs. Both wall thickness and burst pressure of three-layered TEVGs were significantly higher than those of two-layered and single-layered TEVGs (P < 0.05). The two-layered TEVGs could be applied as small-caliber vascular grafts, and three-layered TEVGs could be applied as medium- or large-caliber vascular grafts.

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Acknowledgments

This work was supported by a grant from Science and Technology Committee of Shanghai (09JC1410400).

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Authors and Affiliations

  1. Department of Pediatric Cardiothoracic Surgery, Shanghai Children’s Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, 200127, China

    Ning Ma, Zhenyu Wang, Hao Chen, Yanjun Sun, Haifa Hong, Qi Sun, Meng Yin & Jinfen Liu

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  1. Ning Ma
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  2. Zhenyu Wang
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  3. Hao Chen
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  4. Yanjun Sun
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  5. Haifa Hong
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  6. Qi Sun
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  7. Meng Yin
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  8. Jinfen Liu
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Correspondence to Jinfen Liu.

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Ma, N., Wang, Z., Chen, H. et al. Development of the novel biotube inserting technique for acceleration of thick-walled autologous tissue-engineered vascular grafts fabrication. J Mater Sci: Mater Med 22, 1037–1043 (2011). https://doi.org/10.1007/s10856-011-4257-z

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  • DOI: https://doi.org/10.1007/s10856-011-4257-z

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