Journal of Materials Science: Materials in Medicine

, Volume 21, Issue 11, pp 2999–3008 | Cite as

Effect of scaffold architecture and BMP-2/BMP-7 delivery on in vitro bone regeneration

  • Pinar Yilgor
  • Rui A. Sousa
  • Rui L. Reis
  • Nesrin Hasirci
  • Vasif HasirciEmail author


The aim of this study was to develop 3-D tissue engineered constructs that mimic the in vivo conditions through a self-contained growth factor delivery system. A set of nanoparticles providing the release of BMP-2 initially followed by the release of BMP-7 were incorporated in poly(ε-caprolactone) scaffolds with different 3-D architectures produced by 3-D plotting and wet spinning. The release patterns were: each growth factor alone, simultaneous, and sequential. The orientation of the fibers did not have a significant effect on the kinetics of release of the model protein BSA; but affected proliferation of bone marrow mesenchymal stem cells. Cell proliferation on random scaffolds was significantly higher compared to the oriented ones. Delivery of BMP-2 alone suppressed MSC proliferation and increased the ALP activity to a higher level than that with BMP-7 delivery. Proliferation rate was suppressed the most by the sequential delivery of the two growth factors from the random scaffold on which the ALP activity was the highest. Results indicated the distinct effect of scaffold architecture and the mode of growth factor delivery on the proliferation and osteogenic differentiation of MSCs, enabling us to design multifunctional scaffolds capable of controlling bone healing.


PHBV Bone Marrow MSCs Chitosan Scaffold Tissue Engineer Construct Fiber Organization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This project was conducted within the scope of the EU FP6 NoE Project Expertissues (NMP3-CT-2004-500283). We acknowledge the support to PY through the same project in the form of an integrated PhD grant. We also would like to acknowledge the support from Scientific and Technical Research Council of Turkey (TUBITAK) through project METUNANOBIOMAT (TBAG 105T508).


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Pinar Yilgor
    • 1
  • Rui A. Sousa
    • 2
  • Rui L. Reis
    • 2
  • Nesrin Hasirci
    • 1
    • 3
  • Vasif Hasirci
    • 1
    • 4
    Email author
  1. 1.METU, BIOMAT, Department of BiotechnologyBiotechnology Research UnitAnkaraTurkey
  2. 2.3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, IBB—Institute for Biotechnology and Bioengineering, PT Associated Laboratory, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoGuimaraesPortugal
  3. 3.METU, BIOMAT, Faculty of Arts and Sciences, Department of ChemistryAnkaraTurkey
  4. 4.METU, BIOMAT, Department of Biological SciencesBiotechnology Research UnitAnkaraTurkey

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