Abstract
Dentine carious lesions can be remineralized under optimal conditions, while the surface characteristics of the caries-attacked area may play an important role in the remineralization process. To understand such a surface mechanism, we examined the microstructures of the remineralized area pretreated with different methods. It was found that dentinal tubules on the remineralized surface orientated differently from intrinsic dentine tubules, with the specific alignment angle determined by different surface treatments. Various surface treatments included in this study were 37% phosphoric acid treatment (the etched group), 37% phosphoric acid etching followed by the application of 10% sodium hypochlorite treatment (the deproteinized group), and untreatment (the control group). These findings are helpful for understanding the non-restorative repair of dentine lesions and the remineralization process of the caries-affected dentin surface.
Similar content being viewed by others
References
ten Cate JM. Remineralization of caries lesions extending into dentin. J Dent Res. 2001;80:1407–11. doi:10.1177/00220345010800050401.
Klout B, ten Cate JM. Remineralization of bovine incisor root lesions in vitro: the role of the collagenous matrix. Caries Res. 1991;25:39–45. doi:10.1159/000261340.
Featherstone JD. Fluoride, remineralization and root caries. Am J Dent. 1994;7:271–4.
Lussi A, Linde A. Mineral induction in vivo by dentine proteins. Caries Res. 1993;27:241–8.
He G, George A. Dentin matrix protein 1 immobilized on type I collagen fibrils facilitates apatite deposition in vitro. J Biol Chem. 2004;279:11649–56. doi:10.1074/jbc.M309296200.
Tay FR, Pashley DH. Guided tissue remineralisation of partially demineralised human dentine. Biomaterials. 2008;29:1127–37. doi:10.1016/j.biomaterials.2007.11.001.
Ayad MF. Effects of rotary instrumentation and different etchants on removal of smear layer on human dentin. J Prosthet Dent. 2001;85:67–72. doi:10.1067/mpr.2001.112792.
Pashley DH, Carvalh RM. Dentine permeability and dentine adhesion. J Dent. 1997;24:355–72. doi:10.1016/S0300-5712(96)00057-7.
Inaba D, Ruben J, Takagi O, Arends J. Effect of sodium hypochlorite treatment on remineralization of human dentine in vitro. Caries Res. 1996;30:218–24.
Marshall GW, Balooch M, Kinney JH, Habelitz S, Marshall SJ. Sodium hypochlorite alterations of dentin and dentin collagen. Surf Sci. 2001;491:444–55. doi:10.1016/S0039-6028(01)01310-3.
Lippert F, Parker DM, Jandt KD. In vitro demineralization/remineralization cycles at human tooth enamel surfaces investigated by AFM and nanoindentation. J Colloid Interface Sci. 2004;280:442–8. doi:10.1016/j.jcis.2004.08.016.
Rosales JI, Marshall GW, Marshall SJ, Watanabe LG, Toledano M, Cabrerizo MA, et al. Acid-etching and hydration influence on dentin roughness and wetability. J Dent Res. 1999;28:1554–9. doi:10.1177/00220345990780091001.
Perdigão J, Lopes M, Geraldeli S, Lopes GC, García-Godoy F. Effect of a sodium hypochlorite gel on dentin bonding. Dent Mater. 2000;16:311–23. doi:10.1016/S0109-5641(00)00021-X.
Correr GM, Alonso RCB, Grando MD, Borges AFS, Puppin-Rontani RM. Effect of sodium hypochlorite on primary dentin—a scanning electron microscopy (SEM) evaluation. J Dent. 2006;34:454–9. doi:10.1016/j.jdent.2005.10.003.
El Feninat F, Ellis TH, Sacher E, Stangel I. A tapping mode AFM study of collapse and denaturation in dentinal collagen. Dent Mater. 2000;17:284–8. doi:10.1016/S0109-5641(00)00083-X.
Marshall GW, Wu-Magidi IC, Watanabe LG, Inai N, Balooch M, Kinney JH, et al. Effect of citric acid concentration on dentin demineralization, dehydration and rehydration: atomic force microscopy study. J Biomed Mater Res. 1998;42:500–7.
Mukai Y, ten Cate JM. Remineralization of advanced root dentin lesions in vitro. Caries Res. 2002;36:275–80. doi:10.1159/000063924.
Lynch RJ, ten Cate JM. The effect of adjacent dentine blocks on the demineralisation and remineralisation of enamel in vitro. Caries Res. 2006;40:38–42. doi:10.1159/000088904.
Yamazaki H, Margolis HC. Enhanced enamel remineralization under acidic conditions in vitro. J Dent Res. 2008;87:569–74. doi:10.1177/154405910808700612.
Osorio R, Ceballos L, Tay F, Cabrerizo-Vilchez MA, Toledano M. Effect of sodium hypochlorite on dentin bonding with a polyalkenoic acid-containing adhesive system. J Biomed Mater Res. 2002;60:316–24. doi:10.1002/jbm.10074.
Zavgorodniy AV, Rohanizadeh R, Bulcock S, Swain MV. Ultrastructural observations and growth of occluding crystals in carious detine. Acta Biomater. 2008;4:1427–39. doi:10.1016/j.actbio.2008.04.010.
Acknowledgments
Dr Jiang Tao from the Department of Prosthodontics, College of Dentistry, Wuhan University is kindly acknowledged for his stimulating discussions. Dr Lin Yi from Faculty of Chemistry, Wuhan University is acknowledged for her helpful AFM data analyses. The authors declare no benefit conflict. This investigation was supported by National Science Foundation of China (NSFC, No. 30700957).
Author information
Authors and Affiliations
Corresponding author
Additional information
Yan Liu, Shiqiang Gong authors contributed equally to this paper.
Rights and permissions
About this article
Cite this article
Liu, Y., Gong, S., Yao, L. et al. Slanted orientations of dentine tubules on remineralized dentine surfaces. J Mater Sci: Mater Med 21, 1473–1478 (2010). https://doi.org/10.1007/s10856-010-4027-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10856-010-4027-3