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FGF2-adsorbed macroporous hydroxyapatite bone granules stimulate in vitro osteoblastic gene expression and differentiation

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Abstract

Hydroxyapatite bone granules with a macroporous structure were produced and then adsorbed with basic fibroblast growth factor (FGF2). The in vitro scaffolding role of the granules in cell population and osteogenic differentiation was investigated. The FGF2-adsorbed porous granules allowed the MC3T3-E1 cells to adhere well and then proliferate actively. While the cell growth level on the FGF2-treated granules was observed to be similar to that on the untreated granules, the expression of genes associated with bone, including collagen type I, alkaline phosphatase, and osteocalcin was significantly up-regulated by the FGF2 treatment, particularly at the early stage. Moreover, the production of alkaline phosphatase with prolonged culturing was greatly enhanced on the FGF2-adsorbed granules. Taken together, the FGF2 treatment of the hydroxyapatite granules was effective in the osteogenic development and the FGF2-adsorbed bone granules may be useful in bone regeneration area.

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Acknowledgment

The present research was conducted by the research fund of Dankook University in 2008.

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Authors and Affiliations

  1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea

    Ishik Jeong & Hae-Won Kim

  2. Biomaterials and Tissue Engineering Lab, Department of Nanobiomedical Science and WCU Research Center, Dankook University Graduate School, Cheonan, South Korea

    Hye-Sun Yu, Mi-Kyung Kim & Hae-Won Kim

  3. Department of Biochemistry, College of Medicine, Inha University, Incheon, South Korea

    Jun-Hyeog Jang

  4. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, South Korea

    Hae-Won Kim

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  1. Ishik Jeong
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  2. Hye-Sun Yu
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  5. Hae-Won Kim
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Correspondence to Hae-Won Kim.

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Ishik Jeong and Hye-Sun Yu are contributed equally to this work.

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Jeong, I., Yu, HS., Kim, MK. et al. FGF2-adsorbed macroporous hydroxyapatite bone granules stimulate in vitro osteoblastic gene expression and differentiation. J Mater Sci: Mater Med 21, 1335–1342 (2010). https://doi.org/10.1007/s10856-009-3971-2

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  • DOI: https://doi.org/10.1007/s10856-009-3971-2

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