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Peptide aptamers against titanium-based implants identified through phage display

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Abstract

Commercially pure titanium (cp-Ti) is widely used in the field of long-term clinical oral implantology owing to its ability to allow close bone-implant apposition. The optimization of its function based on artificial proteins has become a key issue in the development of improved cp-Ti implants. Here, we set out to identify peptide aptamers with preferential adsorption towards titanium-based implants through the phage display methodology. Fifteen sequences were selected in the third round of biopanning. One sequence, ATWVSPY (named TBP1), had a 40% repetition rate and exhibited the strongest binding affinity to cp-Ti disks. Ten sequences were selected in the fourth round, among which the repetition rate is 80% for TBP1 and 20% for TBP2 (GVGLPHT). The peptide aptamers against cp-Ti disks can provide an alternative method of functional coating for biomaterial surfaces.

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Acknowledgments

This research was funded by National Science Foundation of China which was conferred to Dr.B.Zhou (NSFC No.30700957). The authors are grateful to the Analytical and Testing Center, Huazhong University of Science and Technology in China for technical assistance.

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Authors and Affiliations

  1. Department of Stomatology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, People’s Republic of China

    Yan Liu, Jing Mao, Bin Zhou, Wei Wei & Shiqiang Gong

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  1. Yan Liu
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  2. Jing Mao
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  3. Bin Zhou
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  4. Wei Wei
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  5. Shiqiang Gong
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Correspondence to Bin Zhou.

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Liu, Y., Mao, J., Zhou, B. et al. Peptide aptamers against titanium-based implants identified through phage display. J Mater Sci: Mater Med 21, 1103–1107 (2010). https://doi.org/10.1007/s10856-009-3970-3

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  • DOI: https://doi.org/10.1007/s10856-009-3970-3

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