Two kinds of insulin were loaded into self-dissolving micropile array tip (following tip). Fully-loaded tip (f-tip) and partially-loaded tip (p-tip) were prepared using chondroitin sulfate for the percutaneous administration of insulin. One hundred micropiles were constructed on a 1.0 × 1.0 cm tip. The mean length of the micropile in a tip were 483.4 ± 4.7 μm for the f-tip and 492.6 ± 2.4 μm for the p-tip. The insulin content of the p-tip was 28.5% of that of the f-tip. The pharmacological efficiency of insulin loaded tip was evaluated in rat experiments by measuring their hypoglycemic effects. The maximum hypoglycemic effect of insulin was observed at 1.7 ± 0.2 h for the f-tip and 1.5 ± 0.2 h for the p-tip. Good dose-dependency was observed for the plasma glucose level vs. time curves. These results suggest the usefulness of p-tip as a percutaneous DDS of insulin.
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This study was supported by a strategic fund of MEXT (Ministry of Education, Culture, Sports, Science and Technology, MEXT) from 2008 to 2010 for establishing research foundations in Japanese private universities. This study was also partially supported by the Uehara Memorial Foundation.
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Ito, Y., Yamazaki, T., Sugioka, N. et al. Self-dissolving micropile array tips for percutaneous administration of insulin. J Mater Sci: Mater Med 21, 835–841 (2010). https://doi.org/10.1007/s10856-009-3923-x
- Stratum Corneum
- Chondroitin Sulfate
- Plasma Glucose Level
- Evans Blue
- Hypoglycemic Effect