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The mesoporosity of microparticles spray dried from trehalose and nanoparticle hydroxyapatite depends on the ratio of nanoparticles to sugar and nanoparticle surface charge

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Abstract

The ratio of hydroxyapatite (HA) nanoparticles (NP) to trehalose in composite microparticle (MP) vaccine vehicles by determining inter-nanoparticle space potentially influences antigen release. Mercury porosimetry and gas adsorption analysis have been used quantify this space. Larger pores are present in MPs spray dried solely from nanoparticle gel compared with MPs spray dried from nanoparticle colloid which have less inter-nanoparticle volume. This is attributed to tighter nanoparticle packing caused by citrate modification of their surface charge. The pore size distributions (PSD) for MP where the trehalose has been eliminated by combustion generally broaden and shifts to higher values with increasing initial trehalose content. Modal pore size, for gel derived MPs is comparable to modal NP width below 30% initial trehalose content and approximates to modal NP length (~50 nm) at 60% initial trehalose content. For colloidally derived MPs this never exceeds the modal NP width. Pore-sizes are comparable, to surface inter-nanoparticle spacings observed by SEM.

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Acknowledgments

This work was funded by a DTI grant (CHBS/004/00063C) held jointly by J. N. Skepper at the University of Cambridge and Cambridge Biostability Ltd. We would like to thank Wayne Hough for his assistance in running samples for Mercury Porosimetry and Dr. Serena Best for access to equipment run within her laboratory.

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Authors and Affiliations

  1. Department of Physiology, Development and Neuroscience, Multi-Imaging Centre School of Biological Sciences, Anatomy Building, Downing Street, Cambridge, CB2 3DY, UK

    David M. Wright, Michael Motskin & Jeremy N. Skepper

  2. Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK

    Zlatko S. Saracevic

  3. Cambridge Biostability Ltd., Unit 184, Cambridge Science Park, Milton Road, Cambridge, Cambridgeshire, CB4 0GA, UK

    Nigel H. Kyle

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  1. David M. Wright
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  2. Zlatko S. Saracevic
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  3. Nigel H. Kyle
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  4. Michael Motskin
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  5. Jeremy N. Skepper
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Correspondence to David M. Wright.

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Wright, D.M., Saracevic, Z.S., Kyle, N.H. et al. The mesoporosity of microparticles spray dried from trehalose and nanoparticle hydroxyapatite depends on the ratio of nanoparticles to sugar and nanoparticle surface charge. J Mater Sci: Mater Med 21, 189–206 (2010). https://doi.org/10.1007/s10856-009-3858-2

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  • DOI: https://doi.org/10.1007/s10856-009-3858-2

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