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A new method for the preparation of bioactive calcium phosphate films hybridized with 1α,25-dihydroxyvitamin D3

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Abstract

The primary goal of this investigation was to develop a calcium phosphate film hybridized with 1α,25-dihydroxyvitamin D3 for the improvement of osteoconductivity of bone substitutes. The hybrid films (hCaP) were prepared at the different concentrations of 1 × 10−10, 1 × 10−8, and 1 × 10−6 M designated as hCaPL, hCaPM, and hCaPH, respectively. The change of the hormone concentration during the preparation of the hybrid films did not cause significant variations on the physical properties of hCaPs, i.e. surface morphology and roughness. On the other hand, X-ray photon spectroscope (XPS) measurements revealed that the concentration change affected the chemical composition of the hybrid films. Recruitment of osteoblast-like MG-63 cells was considerably improved on hCaPs compared to tissue culture plate (TCP). However, cell proliferation on hCaPs was substantially suppressed and inversely proportional to the hormone concentration used. It was observed that bone-like nodules which consisted of bead-like components and well-developed matrix were rapidly formed on hCaPs. Masson’s trichrome and safranin-O stainings elucidated that the bead-like components were MG-63 cells. Safranin-O staining showed that proteoglycan was produced actively. These results indicate that the cells cultured on hCaPs were strongly stimulated by the hormone to produce proteoglycan which can be considered as an induction of premature bone formation. The number of the nodules was increased with hormone concentration and most pronounced at the hCaPH. Gene expression patterns of alkaline phosphatase (ALP), transforming growth factor-β (TGF-β), and osteopontin (OPN) were strongly modulated by hybridized the hormone. For ALP and OPN, gene expressions were activated earlier on hCaPs than untreated calcium phosphate (CaP) confirming the effect of the hybridization was substantial. The TGF-β gene expression was immediately activated after seeding but difference between samples was not significant suggesting that the gene expression was modulated not by the hormone hybridization but by CaP itself. As a result, hybridization of 1,25(OH)2D3 with CaP can be a potentially strong candidate to promote osteoconductivity of implant materials.

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Acknowledgement

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2006-311-D00056).

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  1. Laboratory of Biomaterials and Tissue Engineering, Department of Chemical Engineering, Institute of Tissue Regeneration Engineering, College of Engineering, Dankook University, Jukjeon-dong, Yongin-si, Gyeonggi-do, Korea

    Jae-Young Jung, Yun-Jung Hong & Woo-Kul Lee

  2. Department of Molecular Biology, BK21 Graduate Program for RNA Biology, Institute of Nanosensor and Biotechnology, College of Science, Dankook University, Jukjeon-dong, Yongin-si, Gyeonggi-do, Korea

    Yong Seok Choi & Sunjoo Jeong

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Jung, JY., Hong, YJ., Choi, Y.S. et al. A new method for the preparation of bioactive calcium phosphate films hybridized with 1α,25-dihydroxyvitamin D3 . J Mater Sci: Mater Med 20, 2441–2453 (2009). https://doi.org/10.1007/s10856-009-3817-y

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