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Properties and biocompatibility of chitosan films modified by blending with PVA and chemically crosslinked

Journal of Materials Science: Materials in Medicine volume 20, pages 553–561 (2009)Cite this article

Abstract

In the present work we report the synthesis, characterization, and preliminary biocompatibility of polymer blends based on Chitosan and poly(vinyl alcohol) (PVA) with low degree of hydrolysis and chemically crosslinked by glutaraldehyde for potential application on skin tissue repairing. The microstructure and morphology of the blended hydrogels were characterized through Fourier Transform Infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM/EDX) analysis. Hydrogels were investigated by swelling as preliminary in vitro test using simulated body fluid. In addition, biocompatibility, cytotoxicity, and cell viability were assessed via MTT assay with VERO cell culture and cell spreading-adhesion analysis. It was found that by increasing the chitosan to PVA ratio, simulated body fluid uptake of the material was significantly altered. All the tested hydrogels have clearly presented adequate cell viability, non-toxicity, and suitable properties which can be tailored for prospective use in skin tissue engineering.

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Acknowledgments

The authors acknowledge CNPq/CAPES/FAPEMIG-PMM for financial support on this work. E. S. Costa Jr. thanks CEFET-MG for supporting academic activities during the PhD graduate term. The authors would like to express the gratitude do Prof Dr. Edel F. B. Stancioli for the valuable discussion on biocompatibility assays.

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  1. Department of Metallurgical Engineering and Materials, Federal University of Minas Gerais, Rua Espírito Santo, 35/206-Centro, 30.160-030, Belo Horizonte, MG, Brazil

    Ezequiel de Souza Costa-Júnior, Marivalda M. Pereira & Herman S. Mansur

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  1. Ezequiel de Souza Costa-Júnior
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  2. Marivalda M. Pereira
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  3. Herman S. Mansur
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Correspondence to Herman S. Mansur.

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de Souza Costa-Júnior, E., Pereira, M.M. & Mansur, H.S. Properties and biocompatibility of chitosan films modified by blending with PVA and chemically crosslinked. J Mater Sci: Mater Med 20, 553–561 (2009). https://doi.org/10.1007/s10856-008-3627-7

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  • DOI: https://doi.org/10.1007/s10856-008-3627-7

Keywords

  • Chitosan
  • Simulated Body Fluid
  • VERO Cell
  • Polyvinyl Acetate
  • Chemical Crosslinking