Current treatment strategies for the repair or replacement of bone use synthetic implants with stem cells and their progeny––a new approach to address unmet medical needs. This study has evaluated the effect of a silica-coated bioactive ceramic, namely HASi in comparison to hydroxyapatite (HA) on the adhesion, proliferation and osteogenic differentiation of goat bone marrow-derived mesenchymal stem cells in vitro in a prolonged culture of 28 days. The cellular activities were significantly enhanced on HASi signifying the role of silica to stimulate osteoblast cells. The fabrication of such a ‘cell-ceramic construct using autologous MSCs’ is aimed for the transplantation to a large bone defect site in the goat femur model which still remains a formidable challenge in Orthopedic surgery.
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P.V. Guillot, W. Cui, N.M. Fisk, D.J. Polak, J. Cell Mol. Med. 11, 935–944 (2007)
A.I. Caplan, Tissue Eng. 11 1198–1211 (2005)
N. Jaiswal, S.E. Haynesworth, A.I. Caplan, S.P. Bruder, J. Cell Biochem. 64, 295–312 (1997)
S.L. Ishaug, G.M. Crane, M.J. Miller, A.W. Yasko, M.J. Yaszemski, A.G. Mikos, J. Biomed. Mater. Res. 36, 17–28 (1997)
G.M. Crane, S.L. Ishaug, A.G. Mikos, Nature Med. 1, 1322–1324 (1995)
L.L. Hench, J. Biomed. Mater. Res. 15, 511–518 (1998)
M. Wang, Biomaterials 24, 2133–2151 (2003)
J. Vuola, R. Taurio, H. Goransson, S. Asko-Seljavaara, Biomaterials 19, 223 (1998)
E.M. Carlisle, Science 178, 619 (1972)
A.M. Pietak, J.W. Reid, M.J. Stott, M. Sayer, Biomaterials 28, 4023–4032 (2007)
P.V. Phan, M. Grzanna, J. Chu, A. Polotsky, A. EI-Ghannam, D. Van Heerden, D.S. Hungerford, C.G. Frondoza, J. Biomed. Mater. Res. 67A, 1001–1008 (2003)
M.B. Nair, S.S. Babu, H.K. Varma, A. John, Acta Biomater. 4, 173–181 (2008)
A. John, M.B. Nair, H.K. Varma, A. Bernhardt, M. Gelinsky, Int. J. Appl. Ceram. Technol. 5, 11–19 (2008)
S.M. Mueller, S. Mizuno, L.C. Gersten, J. Glowacki, J. Bone Miner. Res. 14, 2118 (1999)
I. Titorencu, V.V. Jinga, E. Constantinescu, A.V. Gafencu, C. Ciohodaru, I. Manolescu, C. Zaharia, M. Simionescu, Cytotherapy 9, 682–696 (2007)
T. Kizuki, M. Ohgaki, M. Katsura, S. Nakamura, K. Hashimoto, Y. Toda, S. Udagawa, K. Yamashita, Biomaterials 24, 941–947 (2003)
M.A. Malik, D.A. Puleo, R. Bizios, R.H. Doremus, Biomaterials 13, 123–128 (1992)
K.D. Lobel, L.L. Hench, J. Biomed. Mater. Res. 39, 575–579 (1998)
W.N. Kee, T.W.L. David, W.H. Dietmar, Tissue Eng. 11, 182–191 (2005)
M. Hott, B. Noel, D. Bernache-Assolant, C. Rey, P.J. Marie, J. Biomed. Mater. Res. 37, 508–516 (1997)
A. Sepp, R.M. Binns, R.I. Lechler, J. Immunol. Methods 196, 175–180 (1996)
A.J. Salgado, O.P. Coutinho, R.L. Reis, Macromol. Biosci. 4, 743–765 (2004)
S. Sánchez-Salcedo, I. Izquierdo-Barba, D. Arcos, M. Vallet-Regí, Tissue Eng. 12, 279–290 (2006)
P. Valerio, M.M. Pereira, A.M. Goes, M.F. Leite, Biomaterials 25, 2941–2948 (2004)
F.J. Hughes, J.E. Aubin, (Springer US 2007) 1–49
I.D. Xynos, A.J. Edgar, L.D. Buttery, L.L. Hench, J.M. Polak, J. Biomed. Mater. Res. 55, 151–157 (2001)
D.M. Reffitt, N. Ogston, R. Jugdaohsingh, H.F. Cheung, B.A. Evans, R.P. Thompson, J.J. Powell, G.N. Hampson, Bone 32, 127–135 (2003)
The authors thank The Director, SCTIMST and The Head, BMT Wing, for the facilities provided, Mr. Suresh Babu S for material preparation, Mr. R. Sreekumar for SEM images, Dr. T. V. Anilkumar for cLSM, Dr. Sachin J. Shenoy for bone marrow aspiration, and CSIR for Senior Research fellowship for Ms. Manitha and DRDO for financial support.
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Nair, M.B., Varma, H.K. & John, A. Triphasic ceramic coated hydroxyapatite as a niche for goat stem cell-derived osteoblasts for bone regeneration and repair. J Mater Sci: Mater Med 20, 251 (2009). https://doi.org/10.1007/s10856-008-3598-8
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