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Interactions between human neutrophils and mucin-coated surfaces

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Abstract

Recently, we showed microscopically that bovine (BSM), porcine (PGM) and human (MG1) mucin coatings could suppress the adhesion of neutrophils to a polyethylene terephthalate-based model biomaterial (Thermanox). Here, using the release of reactive oxygen species (ROS) as a marker of material-induced neutrophil activation, the strong surface-passivating effects of these mucin coatings were corroborated. Under optimal adsorption conditions, all mucin species performed equally well, thus indicating a high degree of functional homology between the mucins. Cell adhesion and morphology correlated well with the release of ROS. Quartz crystal microbalance (QCM-D) analysis linked low neutrophil activation to efficient mucin surface-shielding. Interestingly, the shielding power appeared equal for thick expanded and thin compact mucin coatings. Combined mucin-serum coatings were found to be highly surface-passivating. Particularly, since our data suggested partly synergistic mucin-serum action, we highlight the possibility that pre-adsorbed mucins could provide favorable support for adsorbing host components.

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Acknowledgements

We greatly acknowledge Prof. Karin Caldwell (Department of Physical and Analytical Chemistry, Uppsala University) for introducing us to the field and providing us with valuable comments during the manuscript preparation. We thank Lena Gröndahl and Riitta Laukkanen-Mållberg (Department of Clinical Chemistry and Pharmacology, Uppsala University) for help with the neutrophil isolation. Leif Ljungh (Department of Medical Cell Biology, Uppsala University) and Dr. Jonas Andersson (Department of Clinical Immunology, Uppsala University) are acknowledged for help with the SEM analysis and the human serum preparation, respectively. Finally, Prof. Per Venge (Department of Clinical Chemistry and Pharmacology, Uppsala University) is acknowledged with gratitude valuable discussions regarding the neutrophil biology.

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  1. Department of Physical and Analytical Chemistry, Division of Surface Biotechnology, BMC, Uppsala University, 751 23, Uppsala, Sweden

    Tomas Sandberg, Jan Carlsson & Marjam Karlsson Ott

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  1. Tomas Sandberg
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  2. Jan Carlsson
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  3. Marjam Karlsson Ott
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Correspondence to Tomas Sandberg.

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Sandberg, T., Carlsson, J. & Karlsson Ott, M. Interactions between human neutrophils and mucin-coated surfaces. J Mater Sci: Mater Med 20, 621–631 (2009). https://doi.org/10.1007/s10856-008-3595-y

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