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Antibacterial protection of suture material by chlorhexidine-functionalized polyelectrolyte multilayer films

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Abstract

The formation of bacterial biofilms on the surface of implanted materials is a critical factor that may lead to chronic microbial infection and tissue necrosis. In the present study we analysed the stability of polyelectrolyte multilayer (ML) films on suture materials and the antibacterial effect obtained with chlorhexidine (CHX)-functionalized films built on different types of suture materials such as silk, polyester and copolymer of glycolide and l-lactide. The comparison of Escherichia coli culture on glass coverslips and glass coverslips with ML and CHX showed at 24 h an inhibition of the bacterial relative luminescence (40.68%, P < 0.5) and at 48 h (99.46%, P < 0.001). In another way, simple soaking of suture material overnight in CHX digluconate 20% without polyelectrolyte films did not at all protect sutures from bacterial colonization but CHX-functionalized polyelectrolyte films, made from poly-l-glutamic acid and poly-l-lysine, inhibited Escherichia coli proliferation.

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Acknowledgements

The authors thank all of the members of INSERM U-595 for their assistance and advice. This work was, in part, financed by the program “Ingenierie tissulaire” by INSERM-CNRS. C.V. thanks the Faculty of Odontology of Strasbourg for its financial support. E.LG. was supported by a doctoral fellowship of the Ministère de l’Enseignement Supérieur et de la Recherche.

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Authors and Affiliations

  1. Faculté de chirurgie dentaire, 1 place de l’Hôpital, 67000, Strasbourg, France

    Jean-Claude Harnet, Henri Tenenbaum & Youssef Haikel

  2. INSERM 595, 11 rue humann, 67000, Strasbourg, France

    Erell Le Guen, Vincent Ball, Joelle Ogier & Constant Vodouhê

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  1. Jean-Claude Harnet
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  2. Erell Le Guen
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  3. Vincent Ball
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  7. Constant Vodouhê
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Correspondence to Constant Vodouhê.

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Youssef Haikel and Constant Vodouhê contributed in an equal way to the management of the project.

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Harnet, JC., Le Guen, E., Ball, V. et al. Antibacterial protection of suture material by chlorhexidine-functionalized polyelectrolyte multilayer films. J Mater Sci: Mater Med 20, 185–193 (2009). https://doi.org/10.1007/s10856-008-3559-2

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