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Coagulation property of hyaluronic acid–collagen/chitosan complex film

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Abstract

Biomacromolecule has been widely used as biomedical material. Because different biomacromolecules possess different properties, how to exhibit the respective advantages of different components on one type of biomaterial becomes the hot spot in the field of biomaterial studying. This work reported a type of complex film that consisted of hyaluronic acid (HA), type I collagen (Col-I), and chitosan (CS) (HA–Col-I/CS, HCC). Then, a series of experiments were performed, such as inverted microscopic observation, atomic force microscopic (AFM) imaging, flow cytometry (FCM) measurement, MTT assay, and MIC assay. In the present work, we observed the growing condition of 3T3 fibroblasts on the surface of the HCC complex film, visualized the morphological changes of platelets during the coagulation process, and discovered microparticles on the platelet membrane. Moreover, we confirmed the microparticles are the platelet-derived microparticles (PMPs) using the FCM. In addition, the minimal inhibitory concentration (MIC) of HCC against Escherichia coli (E. coli) 8099 was 0.025 mg/ml, against Staphylococcus aureus (S. aureus) ATCC 6538 was 0.1 mg/ml. The results together indicated that the HCC film possessed promising coagulation property, cell compatibility and anti-bacteria property, and the potential in future clinical application such as wound healing and bandage.

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References

  1. Q. Feng, G. Zeng, P. Yang et al., Colloid Surf. A 257–258, 85 (2005)

    Article  CAS  Google Scholar 

  2. T. Yamada, T. Kawasaki, J. Biosci. Bioeng. 99, 521 (2005)

    Article  CAS  Google Scholar 

  3. K.M. Stuhlmeier, Wien. Med. Wochenschr. 156, 563 (2006)

    Article  Google Scholar 

  4. W. Choi, H. Kawanabe, Y. Sawa et al., Acta Odontol. Scand. 66, 31 (2008)

    Article  CAS  Google Scholar 

  5. L.J. Alberio, K.J. Clemetson, Curr. Hematol. Rep. 3, 338 (2004)

    Google Scholar 

  6. S. Ilveskero, P. Siljander, R. Lassila, Arterioscler. Thromb. Vasc. Biol. 21, 628 (2001)

    CAS  Google Scholar 

  7. B.P. Chan, K.F. So, J. Biomed. Mater. Res. A 75, 689 (2005)

    CAS  Google Scholar 

  8. L. Ma, C. Gao, Z. Mao et al., Biomaterials 25, 2997 (2004)

    Article  CAS  Google Scholar 

  9. L. Meinel, V. Karageorgiou, S. Hofmann et al., J. Biomed. Mater. Res. A 71, 25 (2004)

    Article  CAS  Google Scholar 

  10. Y. Hu, Y.Z. Wu, J.Y. Cai et al., Int. J. Mol. Sci. 8, 1 (2007)

    Article  CAS  Google Scholar 

  11. J.M. Dang, D.D. Sun, Y. Shin-Ya et al., Biomaterials 27, 406 (2006)

    Article  CAS  Google Scholar 

  12. T. Haque, H. Chen, W. Ouyang et al., Int. J. Artif. Organs 28, 631 (2005)

    CAS  Google Scholar 

  13. G.J. Toes, D.J. van, J. Haan et al., Biomaterials 20, 1951 (1999)

    Article  CAS  Google Scholar 

  14. J. Wang, J.Y. Chen, P. Yang et al., Nucl. Instrum. Meth. B 242, 12 (2006)

    Article  CAS  Google Scholar 

  15. S.N. Rodrigues, I.C. Goncalves, M.C. Martins et al., Biomaterials 27, 5357 (2006)

    Article  CAS  Google Scholar 

  16. P. Fungaloi, E.R. Statius, Y.P. Wu et al., Photochem. Photobiol. 75, 412 (2002)

    Article  CAS  Google Scholar 

  17. C.X. Qu, J.Z. Wang, W.H. Wan et al., J. Clin. Lab. Anal. 20, 250 (2006)

    Article  CAS  Google Scholar 

  18. J.E. Fernandez-Barbero, P. Galindo-Moreno, G. Avila-Ortiz et al., Clin. Oral. Implants Res. 17, 687 (2006)

    Article  Google Scholar 

  19. M.D. Linden, A.L. Frelinger, M.R. Barnard et al., Semin. Thromb. Hemost. 30, 501 (2004)

    Article  Google Scholar 

  20. C. Aurigemma, A. Fattorossi, A. Sestito et al., Thromb. Res. 120, 901 (2007)

    Article  CAS  Google Scholar 

  21. L. Catani, M.E. Fagioli, P.L. Tazzari et al., Exp. Hematol. 134, 879 (2006)

    Article  CAS  Google Scholar 

  22. G. Binnig, C.F. Quate, C. Gerber, Phys. Rev. Lett. 56, 930 (1986)

    Article  Google Scholar 

  23. J.K. Horber, M.J. Miles, Science 302, 1002 (2003)

    Article  CAS  Google Scholar 

  24. J.L. Alonso, W.H. Goldmann, Life Sci. 72, 2553 (2003)

    Article  CAS  Google Scholar 

  25. S. Rammelt, T. Illert, S. Bierbaum et al., Biomaterials 27, 5561 (2006)

    Article  CAS  Google Scholar 

  26. C.A. Siedlecki, R.E. Marchant, Biomaterials 19, 441 (1998)

    Article  CAS  Google Scholar 

  27. M.D. Garrison, B.D. Ratner, Ann. N. Y. Acad. Sci. 831, 101 (1997)

    Article  CAS  Google Scholar 

  28. A. Blinca, J. Magdica, J. Fricb et al., Fibrinolysis Proteolysis 14, 288 (2000)

    Article  Google Scholar 

  29. I. Kang, M. Raghavachari, C.M. Hofmann et al., Thromb. Res. 119, 731 (2007)

    Article  CAS  Google Scholar 

  30. G. Abatangelo, R. Barbucci, P. Brun et al., Biomaterials 18, 1411 (1997)

    Article  CAS  Google Scholar 

  31. C. Jiansu, L. Qinhua, X. Jintang et al., Artifi. Organs 29, 104 (2005)

    Article  Google Scholar 

  32. J.S. Mao, H.F. Liu, Y.J. Yin et al., Biomaterials 24, 1621 (2003)

    Article  CAS  Google Scholar 

  33. T. Tanabe, N. Okitsu, A. Tachibana et al., Biomaterials 23, 817 (2002)

    Article  CAS  Google Scholar 

  34. M. Kanno, H. Kawakami, S. Nagaoka et al., J. Biomed. Mater. Res. 60, 53 (2002)

    Article  CAS  Google Scholar 

  35. Y. Yano, J. Kambayashi, E. Shiba et al., Biochem. J. 299(Pt 1), 303 (1994)

    CAS  Google Scholar 

  36. A. Vidovic, M. Vilibic, A. Markotic et al., Psychiatry Res. 150, 211 (2007)

    Article  CAS  Google Scholar 

  37. T. Kalsch, E. Elmas, X.D. Nguyen et al., Thromb. Res. 120, 703 (2007)

    Article  CAS  Google Scholar 

  38. V. Serebruany, A. Malinin, A. Pokov et al., Thromb. Res. 119, 175 (2007)

    Article  CAS  Google Scholar 

  39. T. Kalsch, X.D. Nguyen, E. Elmas et al., Int. J. Cardiol. 111, 217 (2006)

    Article  Google Scholar 

  40. C.A. Siedlecki, I.W. Wang, J.M. Higashi et al., Biomaterials 20, 1521 (1999)

    Article  CAS  Google Scholar 

  41. S. Cauwenberghs, M.A. Feijge, A.G. Harper et al., FEBS Lett. 580, 5313 (2006)

    Article  CAS  Google Scholar 

  42. B. Li, J.F. Kennedy, J.L. Penga et al., Carbohydr. Polym. 65, 488 (2006)

    Article  CAS  Google Scholar 

  43. X. Wang, Y. Du, J. Yang et al., J. Biomed. Mater. Res. A 84, 384 (2008)

    Google Scholar 

  44. L. Nan, C. XiGuang, P. HyunJin et al., Carbohydr. Polym. 64, 60 (2006)

    Article  CAS  Google Scholar 

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Acknowledgments

We thank associate professor Meiying Zhang (Biomedicine Research & Development Center, Jinan University) for her warm help in cell culture techniques. This work is supported by the NSFC (No. 60578025, 30540420311).

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Authors and Affiliations

  1. Department of Chemistry, Jinan University, Guangzhou, 510632, People’s Republic of China

    Yangzhe Wu, Yi Hu & Jiye Cai

  2. The First Affiliated Hospital, Jinan University, Guangzhou, 510632, People’s Republic of China

    Shuyuan Ma & Xiaoping Wang

Authors
  1. Yangzhe Wu
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  2. Yi Hu
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  3. Jiye Cai
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  4. Shuyuan Ma
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  5. Xiaoping Wang
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Corresponding author

Correspondence to Jiye Cai.

Additional information

Yangzhe Wu and Yi Hu contributed equally to this work.

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Wu, Y., Hu, Y., Cai, J. et al. Coagulation property of hyaluronic acid–collagen/chitosan complex film. J Mater Sci: Mater Med 19, 3621–3629 (2008). https://doi.org/10.1007/s10856-008-3477-3

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  • DOI: https://doi.org/10.1007/s10856-008-3477-3

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