Skip to main content
SpringerLink
Log in

Effects of human hair and nail proteins and their films on rat mast cells

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Human hair and nail are valuable materials for producing individual corresponding biocompatible materials. A rapid and convenient protein extraction method (Shindai method) and novel procedures for preparing their protein films from their extracts have been developed using human hair and nail. The effects of the human hair and nail proteins and their films on histamine release from rat peritoneal mast cells were investigated. Both protein solutions and their films, mainly consisting of keratins and matrix proteins, did not induce histamine release from the mast cells. Scanning electron microscopy (SEM) also showed that the mast cells were only slightly affected by adding the human hair and nail proteins or by incubating on their protein films. The IgE-dependent histamine release was inhibited by the hair and nail proteins and their films. Incubation of the mast cells with the hair and nail proteins prior to the addition of the IgE serum resulted in a high inhibition (50%) of the histamine release, while the inhibition was approximately 10% when the protein solutions were mixed with the mast cells after incubation with the IgE serum. These results suggest that the human hair and nail proteins and their films will be useful materials for antiallergic actions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. A. NAKAMURA, M. ARIMOTO, K. TAKEUCHI and T. FUJII, Biol. Pharm. Bull. 25, 569 (2002)

    Article  CAS  Google Scholar 

  2. T. FUJII and S. MURAI, Kobunshi Ronbunshu 62, 201 (2005)

    CAS  Google Scholar 

  3. T. FUJII, D. OGIWARA and M. ARIMOTO, Biol. Pharm. Bull. 27, 89 (2004)

    Article  CAS  Google Scholar 

  4. T. FUJII and Y. IDE, Biol. Pharm. Bull. 27, 1433 (2004)

    Article  CAS  Google Scholar 

  5. K. YAMAUCHI, M. MANIWA and T. MORI, J. Biomater. Sci. Polym. Ed. 9, 259 (1998)

    CAS  Google Scholar 

  6. A. TACHIBANA, Y. FURUTA, H. TAKESHIMA, T. TANABE and K. YAMAUCHI, J. Biotech. 93, 165 (2002)

    Article  CAS  Google Scholar 

  7. Y. IDE and T. FUJII, Sen’I Gakkishi 60, 276 (2004)

    Article  CAS  Google Scholar 

  8. K. YAMAUCHI, A. YAMAUCHI, T. KUSUNOKI, A. KHODA and Y. KONISHI, J. Biomed. Mater. Res. 31, 439 (1996)

    Article  CAS  Google Scholar 

  9. Y. IDE and T. FUJII, Kobunshi Ronbunshu 61, 153 (2004)

    Google Scholar 

  10. T. ISHIZAKA and K. ISHIZAKA, Prog. Allergy 34, 188 (1984)

    CAS  Google Scholar 

  11. S. M. MIESCHER and M. VOGEL, Mol. Aspects Med. 23, 413 (2002)

    Article  CAS  Google Scholar 

  12. L. J. WALSH, Crit. Rev. Oral Biol. Med. 14, 188 (2003)

    Article  Google Scholar 

  13. T. FUJII, H. YOKOYAMA, G. F. LI and H. TADA, Biol. Pharm. Bull. 20, 79 (1997)

    CAS  Google Scholar 

  14. U. K. LAEMMLI, Nature 227, 680 (1970)

    Article  CAS  Google Scholar 

  15. M. M. BRADFORD, Anal. Biochem. 72, 248 (1976)

    Article  CAS  Google Scholar 

  16. P. A. SHORE, A. BURKHALTER and V. H. COHN Jr, J. Pharmacol. Exp. Ther. 127, 182 (1959)

    CAS  Google Scholar 

  17. K. IZUSHI and K. TASAKA, Immunopharmacol. 18, 177 (1989)

    Article  CAS  Google Scholar 

  18. B. JANSANI, G. KREIL, B. F. MACKLER and D. R. STANWORTH, Biochem. J. 181, 623 (1979)

    Google Scholar 

  19. A. ARGIOLAS and J. J. PISANO, J. Biol. Chem. 260, 1437 (1990)

    Google Scholar 

  20. H. W. HEID, E. WERNER and W. W. FRANKE, Differentiation 32, 101 (1986)

  21. L. LANGBEIN, M. A. ROGERS, H. WINTER, S. PRAETZEL, U. BECKHAUS, H. R. RACKWITZ and J. SCHWEIZER, J. Biol. Chem. 274, 19874 (1999)

    Article  CAS  Google Scholar 

  22. L. LANGBEIN, M. A. ROGERS, H. WINTER, S. PRAETZEL and J. SCHWEIZER, J. Biol. Chem. 276, 35123 (2001)

    Article  CAS  Google Scholar 

  23. M. A. ROGERS, H. WINTER, L. LANGBEIN, C. WOLF and J. SCHWEIZER, J. Invest. Dermatol. 114, 464 (2000)

    Article  CAS  Google Scholar 

  24. Y. HIRAO, K. OHKAWA, H. YAMAMOTO and T. FUJII, Macromol. Mater. Eng. 290, 165 (2005)

    Article  CAS  Google Scholar 

  25. S. KOBAYASHI, H. MORIKAWA, S. ISHII and T. FUJII, JSME Int. J. Ser. C. 48, 494 (2005)

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported in part by Grants-in-Aid for Science Research (B) (16350123) and CLUSTER (the second stage) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We are also indebted to the Division of Gene Research Center, Research Center for Human and Environmental Science, Shinshu University, for providing the facilities.

Author information

Authors and Affiliations

  1. Department of Kansei Engineering, Faculty of Textile Science and Technology, Shinshu University, Tokida 3-15-1, Ueda, 386-8567, Japan

    Toshihiro Fujii & Shinya Murai

  2. Institute of High Polymer Research, Faculty of Textile Science and Technology, Shinshu University, Tokida 3-15-1, Ueda, 386-8567, Japan

    Kousaku Ohkawa

  3. Department of Materials Chemistry, Faculty of Textile Science and Technology, Shinshu University, Tokida 3-15-1, Ueda, 386-8567, Japan

    Toshihiro Hirai

Authors
  1. Toshihiro Fujii
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shinya Murai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kousaku Ohkawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Toshihiro Hirai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Toshihiro Fujii.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fujii, T., Murai, S., Ohkawa, K. et al. Effects of human hair and nail proteins and their films on rat mast cells. J Mater Sci: Mater Med 19, 2335–2342 (2008). https://doi.org/10.1007/s10856-007-3341-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-007-3341-x

Keywords

Search

Navigation