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Dose-dependent effects of Ni (II) ions on production of three inflammatory cytokines (TNF-α, IL-1β and IL-6), superoxide dismutase (SOD) and free radical NO by murine macrophage-like RAW264 cells with or without LPS-stimulation

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Abstract

The effect of Ni (II) ions on macrophages is not well understood. The purpose of this study was to examine the dose-dependent effects of Ni (II) ions up to 1,000 μmol/L on production of three inflammatory cytokines (TNF-α, IL-1β and IL-6), superoxide dismutase (SOD) and nitric oxide (NO) by murine macrophage-like RAW264 cells with (+) or without (−) lipopolysaccharide (LPS) -stimulation. Ni (II) ions caused LPS (−) RAW264 cells to slightly increase production of TNF-α and IL-6, proportionally to the Ni (II) ion concentration while IL-1β was not produced, and to slightly increase production of SOD and NO. It can be concluded that Ni (II) ions dose-dependently increased the inflammatory and oxidative stress conditions of LPS (−) RAW264 cells. LPS-stimulation caused RAW264 cells to produce in abundance the three inflammatory cytokines, SOD and NO. Ni (II) ions dose-dependently reduced the three cytokine quantities and NO amounts in LPS (+) RAW264 cells, while dose-independently increasing SOD amounts. It was noted that Ni (II) ions dose-dependently reduce the resistance power against bacteria of LPS (+) macrophages, because the production of volatile NO—bacteria killer is diminished proportionally to the Ni (II) ion concentration.

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References

  1. C. S. JENSEN, S. LISBY, O. BAADSGAARD, K. BYRIALSEN and T. MENNE, Contact Derm. 172 (2003) 1926

    Google Scholar 

  2. Y. YONEKURA, K. EBDO, M. IIJIMA, H. OHNO and I. MIZOGUCHI, Dent. Mater. J. 23 (2004) 197

    Google Scholar 

  3. D. L. EDWARDS, J. C. WATAHA and C. T. HANKS, J. Oral Rehabil. 25 (1998) 2

    Article  CAS  Google Scholar 

  4. J. G. DUGUID and V. A. BLOOMFIELD, Biophys. J. 69 (1995) 2642

    CAS  Google Scholar 

  5. J. B. LEWIS, R. L. MESSER, V. V. MCCLOUD, P. E. LOCKWOOD, S. D. HSU and J. C. WATAHA, Biomaterials 27 (2006) 5348

    Article  CAS  Google Scholar 

  6. S. KAWANISHI, S. OIKAWA, S. INOUE and K. NISHINO, Environ. Health Perspect. 110 (2002) 789

    CAS  Google Scholar 

  7. K. S. KASPRZAK, F. W. SUNDERMAN and K. SALNIKOW, Mutat. Res. 533 (2003) 67

    CAS  Google Scholar 

  8. G. SCHMALZ, U. SCHSTER and H. SCHWIKL, Biomaterials 19 (1998) 1689

    Article  CAS  Google Scholar 

  9. M. TAIRA, M. SASAKI, C. YAMAURA-TANAKA, S. SAITOH, S. KIMURA and Y. ARAKI, Dent. Mater. J. 24 (2005) 304

    CAS  Google Scholar 

  10. H. L. KIM, H. S. LEE, Y. H. CHONG and J. L. KANG, Toxicology 225 (2006) 36

    Article  CAS  Google Scholar 

  11. D. R. RAKICH, J. C. WATAHA, C. A. LEFEBVRE and R. N. WELLER, J. Endod. 25 (1999) 114

    Article  CAS  Google Scholar 

  12. L. SHAPIRA, W. A. SOSKOLNE, M. N. SELA, S. OFFENBACHER and V. BARAK, J. Periodontolol. 65 (1994) 139

    CAS  Google Scholar 

  13. V. C. CULOTTA, M. YANG and T. V. O’HALLORAN, Biochim. Biophys. Acta. 1763 (2006) 747

    Article  CAS  Google Scholar 

  14. S. OILSHEVSKY, A. BURLAKA, E. SIDORIK, V. SHLYAKHOVENKO, Y. GARPENKO and V. KOZAK, Exp. Oncol. 28 (2006) 114

    Google Scholar 

  15. P. K. VADIVELOO, E. KERAMIDARIS, W. A. MORRISON and A. G. STEWART, Biochim. Biophys. Acta. 1539 (2001) 140

    Article  CAS  Google Scholar 

  16. M. NODA, J. C. WATAHA, P. E. LOCKWOOD, K. R. VOLKMANN, M. KAGA and H. SANO, Dent. Mater. 19 (2003) 101

    Article  CAS  Google Scholar 

  17. M. SASAKI, Y. OHARA-NEMOTO, S. TAJIKA, M. KOBAYASHI, C. YAMAURA and S. KIMURA, J. Med. Microbiol. 50 (2001) 952

    CAS  Google Scholar 

  18. M. T. COSTA, M. A. LENZA, C. S. GOSCH, I. COSTA and F. RIBEIRO-DIAS, J. Dent. Res. 86 (2007) 441

    Article  CAS  Google Scholar 

  19. J. C. WATAHA, P. E. LOCKWOOD, S. S. KHAJOTIA and R. TURNER, J. Prosthet. Dent. 80 (1998) 691

    Article  CAS  Google Scholar 

  20. J. C. WATAHA, S. RATANASATHIEN, C. T. HANKS and Z. SUN, Dent. Mater. 12 (1996) 322

    Article  CAS  Google Scholar 

  21. M. BERGMAN, B. BERGMAN and R. SOREMARK, J. Oral Rehabil. 7 (1980) 325

    Article  CAS  Google Scholar 

  22. M. IIJIMA, K. ENDO, H. OHNO, Y. YONEKURA and I. MIZOGUCHI, Dent. Mater. J. 20 (2001) 103

    CAS  Google Scholar 

  23. J. W. BAUMAN, J. LIU and C. D. KLAASSEN, Fundam. Appl. Toxicol. 21 (1993) 15

    Article  CAS  Google Scholar 

  24. N. VERNEUIL, A. MAZE, M. SANGUINETTI, J. M. LAPLACE, A. BENACHOUR, Y. AUFFRAY, J. C. GIARD and A. HARTKE, Microbiology 152 (2006) 2579

    Article  CAS  Google Scholar 

  25. M. TAIRA, M. SASAKI, S. KIMURA and Y. ARAKI. in “International Congress Series 1284 Oral Health, 2005 September”, Edited by: N. WATANABE, N. TAKAHASHI and H. TAKADA (Elsevier B.V., Amsterdam, 2005) p. 205

  26. J. B. LEWIS, J. C. WATAHA, V. MCCLOUD, P. E. LOCKWOOD, R. L. MESSER and W. Y. TSENG, J. Biomed. Mater. Res. A. 74 (2005) 474

    CAS  Google Scholar 

  27. A. V. KOZLOV, L. GILLE, I. MILLER, C. PISKERNIK, S. HAINDL, K. STANIEK, H. NOHL, S. BAHRAMI, W. OHLINGER, M. GEMEINER and H. RED, Biochem. Biophys. Res. Commun. 352 (2007) 91

    Article  CAS  Google Scholar 

  28. Y. H. LI, Z. Q. BRAUNER and K. TULLUS, Pediatr. Res. 49 (2001) 820

    Article  CAS  Google Scholar 

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Acknowledgments

This study was supported in part by (1, 2) Grants-in-Aid (B) 18390522 and (Exploratory Research) 19659511 by the Japan Society for the Promotion of Science and (3, 4) Grants-in-Aid for High-performance Biomedical Materials Research from 2005 to 2009 and for the Open Research project from 2007 to 2011 from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, Morioka, Iwate, 020-8505, Japan

    Masayuki Taira & Yoshima Araki

  2. Department of Oral Microbiology, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, Morioka, 020-8505, Japan

    Minoru Sasaki & Shigenobu Kimura

Authors
  1. Masayuki Taira
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  2. Minoru Sasaki
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  3. Shigenobu Kimura
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  4. Yoshima Araki
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Correspondence to Masayuki Taira.

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Taira, M., Sasaki, M., Kimura, S. et al. Dose-dependent effects of Ni (II) ions on production of three inflammatory cytokines (TNF-α, IL-1β and IL-6), superoxide dismutase (SOD) and free radical NO by murine macrophage-like RAW264 cells with or without LPS-stimulation. J Mater Sci: Mater Med 19, 2173–2178 (2008). https://doi.org/10.1007/s10856-007-3322-0

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  • DOI: https://doi.org/10.1007/s10856-007-3322-0

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