Skip to main content

Development, optimization and characterization of a full-thickness tissue engineered human oral mucosal model for biological assessment of dental biomaterials

Journal of Materials Science: Materials in Medicine volume 19, pages 1793–1801 (2008)Cite this article

Abstract

Restorative dental materials and oral health care products come into direct contact with oral mucosa and can cause adverse reactions. In order to obtain an accurate risk assessment, the in vitro test model must reflect the clinical situation as closely as possible. The aim of this study was to develop and optimize a three-dimensional full-thickness engineered human oral mucosal model, which can be used for biological assessment of dental materials. In this study human oral fibroblasts and keratinocytes were isolated from patients and seeded onto a number of collagen-based and synthetic scaffolds using a variety of cell seeding techniques and grown at the air/liquid interface to construct human oral mucosa equivalents. Suitability of 10 different scaffolds for engineering human oral mucosa was evaluated in terms of biocompatibility, biostability, porosity, and the ability to mimic normal human oral mucosa morphology. Finally an optimized full-thickness engineered human oral mucosa was developed and characterized using transmission electron microscopy and immunostaining. The oral mucosa reconstruct resembled native human oral mucosa and it has the potential to be used as an accurate and reproducible test model in mucotoxicity and biocompatibility evaluation of dental materials.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. C. AUGUSTIN, C. COLLOMBEL and O. DAMOUR, Photodermatol. Photoimmunol. Photomed. 13 (1997) 27

    CAS  Google Scholar 

  2. C. AUGUSTIN, V. FREI, E. PERRIER, A. HUC and O. DAMOUR, Skin Pharmacol. 10 (1997) 63

    CAS  Google Scholar 

  3. J. HOLLE and R. KUNSTFELD, Plast. Reconstr. Surg. 114 (2004) 599; author reply 599

    Article  Google Scholar 

  4. G. SAINTIGNY, M. BONNARD, O. DAMOUR and C. COLLOMBEL, Acta Derm. Venereol. 73 (1993) 175

    CAS  Google Scholar 

  5. M. OKAZAKI, K. YOSHIMURA, Y. SUZUKI and K. HARII, Plast. Reconstr. Surg. 112 (2003) 784

    Article  Google Scholar 

  6. M. MERNE and S. SYRJANEN, Arch. Dermatol. Res. 295 (2003) 190

    Article  CAS  Google Scholar 

  7. I. MACKENZIE, G. RITTMAN, A. BOHNERT, D. BREITKREUTZ and N. E. FUSENIG, Epithelial Cell Biol. 2 (1993) 107

    CAS  Google Scholar 

  8. K. MOHARAMZADEH, I. M. BROOK, R. VAN NOORT, A. M. SCUTT and M. H. THORNHILL, J. Dent. Res. 86 (2007) 115

    CAS  Article  Google Scholar 

  9. I. MASUDA, Kokubyo Gakkai Zasshi 63 (1996) 334

    CAS  Google Scholar 

  10. T. MORIYAMA, I. ASAHINA, M. ISHII, M. ODA, Y. ISHII and S. ENOMOTO, Tissue Eng. 7 (2001) 415

    Article  CAS  Google Scholar 

  11. M. ROUABHIA, N. DESLAURIERS, Biochem. Cell Biol. 80 (2002) 189

    Article  CAS  Google Scholar 

  12. K. H. CHO, H. T. AHN, K. C. PARK, J. H. CHUNG, S. W. KIM, M. W. SUNG, K. H. KIM, P. H. CHUNG, H. C. EUN and J. I. YOUN, J. Dermatol. Sci. 22 (2000) 117

    Article  CAS  Google Scholar 

  13. S. BHARGAVA, C. R. CHAPPLE, A. J. BULLOCK, C. LAYTON and S. MACNEIL, BJU Int. 93 (2004) 807

    Article  CAS  Google Scholar 

  14. K. IZUMI, G. TAKACS, H. TERASHI and S. E. FEINBERG, J. Oral Maxillofac Surg. 57 (1999) 571

    Article  CAS  Google Scholar 

  15. H. T. RUPNIAK, C. ROWLATT, E. B. LANE, J. G. STEELE, L. K. TREJDOSIEWICZ, B. LASKIEWICZ, S. POVEY and B. T. HILL, J. Natl. Cancer Inst. 75 (1985) 621

    CAS  Google Scholar 

  16. K. TAKEDA, A. GOSIEWSKA and B. PETERKOFSKY, J. Cell. Physiol. 153 (1992) 450

    Article  CAS  Google Scholar 

  17. M. R. KHORRAMIZADEH, E. E. TREDGET, C. TELASKY, Q. SHEN and A. GHAHARY, Mol. Cell. Biochem. 194 (1999) 99

    Article  CAS  Google Scholar 

  18. L. MA, C. GAO, Z. MAO, J. SHEN, X. HU and C. HAN, J. Biomater. Sci. Polym. Ed. 14 (2003) 861

    Article  CAS  Google Scholar 

  19. F. YANG, R. MURUGAN, S. WANG and S. RAMAKRISHNA, Biomaterials 26 (2005) 2603

    Article  CAS  Google Scholar 

  20. X. ZONG, H. BIEN, C. Y. CHUNG, L. YIN, D. FANG, B. S. HSIAO, B. CHU and E. ENTCHEVA, Biomaterials 26 (2005) 5330

    Article  CAS  Google Scholar 

  21. H. K. KLEINMAN and G. R. MARTIN, Semin. Cancer Biol. 15 (2005) 378

    Article  CAS  Google Scholar 

  22. A. F. BLACK, C. BOUEZ, E. PERRIER, K. SCHLOTMANN, F. CHAPUIS and O. DAMOUR, Tissue Eng. 11 (2005) 723

    Article  CAS  Google Scholar 

  23. L. MA, C. GAO, Z. MAO, J. ZHOU, J. SHEN, X. HU and C. HAN, Biomaterials 24 (2003) 4833

    Article  CAS  Google Scholar 

  24. L. SHAHABEDDIN, F. BERTHOD, O. DAMOUR and C. COLLOMBEL, Skin Pharmacol. 3 (1990) 107

    CAS  Article  Google Scholar 

  25. J. MA, H. WANG, B. HE and J. CHEN, Biomaterials 22 (2001) 331

    Article  CAS  Google Scholar 

  26. K. VON FIGURA, Hautarzt 27 (1976) 206

    CAS  Google Scholar 

  27. J. P. KIM, J. D. CHEN, M. S. WILKE, T. J. SCHALL and D. T. WOODLEY, Lab. Invest. 71 (1994) 401

    CAS  Google Scholar 

  28. F. BERTHOD, D. HAYEK, O. DAMOUR and C. COLLOMBEL, Biomaterials 14 (1993) 749

    Article  CAS  Google Scholar 

Download references

Acknowledgement

The authors would like to acknowledge Professor Paul Hatton, Mrs Jen Mundy, Dr. Aileen Crawford, and Dr. Kathryn Hurrell-Gillingham for their advice and assistance.

Author information

Authors and Affiliations

  1. Center for Biomaterials and Tissue Engineering, School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield, S10 2TA, UK

    K. Moharamzadeh, I. M. Brook, R. Van Noort, A. M. Scutt, K. G. Smith & M. H. Thornhill

Authors
  1. K. Moharamzadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. M. Brook
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Van Noort
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. M. Scutt
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. G. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. H. Thornhill
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. Moharamzadeh.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Moharamzadeh, K., Brook, I.M., Van Noort, R. et al. Development, optimization and characterization of a full-thickness tissue engineered human oral mucosal model for biological assessment of dental biomaterials. J Mater Sci: Mater Med 19, 1793–1801 (2008). https://doi.org/10.1007/s10856-007-3321-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-007-3321-1

Keywords

  • PLLA
  • Oral Mucosa
  • Oral Epithelium
  • Cell Culture Insert
  • Normal Oral Mucosa