Abstract
In recent experimental studies, we could demonstrate the occurrence of antibodies against the prosthesis matrix and coating following implantation of polyester-based vascular grafts. Therefore, this study aimed at evaluating the biocompatibility of a new absorbable polymer coating by detection of antibodies against the coating and the polyester matrix. Two polyester vascular prostheses coated either with the polymer (PP-prosthesis) or with gelatine (PG-prosthesis) were functionally implanted into sheep (n = 22 per group). Blood was drawn on days 1 (pre-OP) and 7, 14, 28, 56, 84, 140 (post-OP). Homogenates from both prostheses (PP-target or PG-target) or from an uncoated prosthesis (P-target) were used as assay targets in a particle-based immunoassay. The antibody binding against the P-target was significantly higher in the PP-group than in the PG-group on days 7–56, but not on days 84 and 140. Within both groups, no significant differences but a significant correlation between the binding against the P-target and the coated target was found. Therefore, the absorbable polymer did not induce a specific humoral immune response. In conclusion, the overall immunogenicity of the polymer-coated graft was comparable to the gelatine-coated graft. The detection of prosthesis-specific antibodies seems to be useful for in vivo biocompatibility testing.
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L. TANG and J. W. EATON, Am. J. Clin. Pathol. 103 (1995) 466
K. YAMAMOTO, Y. NOISHIKI, M. MO, J. KONDO and A. MATSUMOTO, Artif. Organs 17 (1993) 1010
K. S. WEADOCK and J. A. GOGGINS, J. Long. Term. Eff. Med. Implants 3 (1993) 207
Z. XIA and J. T. TRIFFITT, Biomed. Mater. 1 (2006) R1
L. TANG, T. A. JENNINGS and J. W. EATON, Proc. Natl. Acad. Sci. USA 95 (1998) 8841
C. DAVIS, J. FISCHER, K. LEY and I. J. SAREMBOCK, J. Thromb. Haemost. 1 (2003) 1699
J. M. ANDERSON, in “Biomaterials Science. An Introduction to Materials in Medicine” (Elsevier Academic Press, San Diego, 2004) p. 299
L. NORGREN, S. HOLTAS, H. PERSSON, E. RIBBE, T. SAXNE and J. THÖRNE, Eur. J.Vasc. Surg. 4 (1990) 379
THE CANADIAN MULTICENTER HEMASHIELD STUDY GROUP, J. Vasc. Surg. 12 (1990) 741
R. M. GOLDBLUM, R. P. PELLEY, A. A. O’DONELL, D. PYRON and J. P. HEGGERS, Lancet 340 (1992) 510
W. H. DE JONG, M. KALLEWAARD, C. A. GOLDHOORN, C. M. VERHOEF, J. W. BIJLSMA, J. S. SCHOUTEN and H. VAN LOVEREN, Biomaterials 25 (2004) 1095
R. ZIPPEL, L. WILHELM, F. MARUSCH, A. KOCH, G. URBAN and M. SCHLOSSER, Eur. J. Vasc. Endovasc. Surg. 21 (2001) 202
M. SCHLOSSER, L. WILHELM, G. URBAN, B. ZIEGLER, M. ZIEGLER and R. ZIPPEL, J. Biomed. Mater. Res. 61 (2002) 450
L. WILHELM, R. ZIPPEL, T. VON WOEDTKE, H. KENK, A. HOENE, M. PATRZYK and M. SCHLOSSER, J. Biomed. Mater. Res. A 83 (2007) 104
M. SCHLOSSER, R. ZIPPEL, A. HOENE, G. URBAN, T. UEBERRUECK, F. MARUSCH, A. KOCH, L. MEYER and L. WILHELM, J. Biomed. Mater. Res. A 72 (2005) 317
R. ZIPPEL, L. WILHELM, A. HOENE, U. WALSCHUS, T. UEBERRUECK and M. SCHLOSSER, J. Biomed. Mater. Res. B (in press). doi:10.1002/jbm.b.30951
Acknowledgments
We are grateful to Kirsten Tornow for excellent technical assistance. The study was supported by AESCULAP AG & Co. KG, Tuttlingen, Germany.
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Walschus, U., Goldmann, H., Ueberrueck, T. et al. Evaluation of the biocompatibility of a new vascular prosthesis coating by detection of prosthesis-specific antibodies. J Mater Sci: Mater Med 19, 1595–1600 (2008). https://doi.org/10.1007/s10856-007-3316-y
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DOI: https://doi.org/10.1007/s10856-007-3316-y